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Targeted Mutagenesis in Mice: A Video Guide


Subject Area(s):  Developmental BiologyMultimediaMouse BiologyLaboratory Techniques

By Roger A. Pedersen, University of California, San Francisco; Virginia Papaioannou, Columbia University
 

© 1993 • 70-minute video
PAL • $210 42.00 (click here to price in UK Pounds)
ISBN  978-087969430-0
You save: 80%

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Description

Embryonic stem (ES) cells are important vehicles for transfer of genes into mice. When combined with normal pre-implantation embryos and transferred to foster mothers, ES cells contribute to the development of many different tissues, including the germline. When these cells are genetically manipulated, this approach discloses much about the function of specific genes.

Detailed methods for using ES cells are described in the manual Manipulating the Mouse Embryo: A Laboratory Manual, Second Edition.

This video guide complements Manipulating the Mouse Embryo: A Laboratory Manual, Second Edition and Transgenic Techniques in Mice: A Video Guide. It provides step-by-step demonstrations of the following procedures required for making mutant mice.

Contents

Demonstration 1: Starting embryonic stem (ES) cell lines
Demonstration 2: Making fibroblast feeder layers
Demonstration 3: Growing established ES cell lines
Demonstration 4: Targeting ES cell lines
Demonstration 5: Making chimeras with ES cells
Demonstration 6: Making a mouse