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Antibodies: A Laboratory Manual, Second edition


Subject Area(s):  BiotechnologyMolecular BiologyBiochemistryLaboratory Techniques

Edited by Edward A. Greenfield, Dana-Farber Cancer Institute

Download Free Excerpts from Antibodies: A Laboratory Manual, Second Edition:

Download Preface and Contents
Download Introduction to Chapter 7, Part 1
Download Introduction to Chapter 7, Part 2
Download Index



© 2014 • 847 pp., illus. (32 4C, 103 B&W), index
Paperback • $175 122.50 (click here to price in UK Pounds)
ISBN  978-1-936113-81-1
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Description

It has been 25 years since the now classic lab manual Antibodies, by Harlow and Lane appeared. This second edition of Antibodies, edited by Edward A. Greenfield of the Dana-Farber Cancer Institute builds on the core strengths of the first edition, presenting clear and authoritative protocols with extensive background information and troubleshooting advice. The original introductory chapters have been recast and updated to take into account our current understanding of the immune system. The critical chapters on generating monoclonal antibodies and growing hybridomas, which demystified hybridoma generation, have been greatly expanded and updated to make these procedures easy to follow and adaptable to current research needs. The remaining chapters contain all new protocols and topics and reflect the progress in how antibodies are studied and used since the appearance of the original edition. These include:
  • antibody purification and storage
  • engineering antibodies, including use of degenerate oligonucleotides, 5’-RACE, phage display, and mutagenesis
  • extensive labeling techniques
  • new immunoblotting protocols
  • the latest screening and labeling techniques
As Dr. Greenfield notes in his preface to this second edition: "The Antibodies manual provided our laboratory with guidance in the form of protocols and recommendations for setting up a hybridoma facility. Everything we needed to know to make a monoclonal antibody was all there, neatly packaged in an easy-to-understand book….the second edition is intended to provide the necessary information and protocols to assist investigators with their first monoclonal antibody effort as well as to provide guidance for more experiences antibody makers who are having some difficulties with a particular project."

Contents

Preface
1 Antibody Production by the Immune System
Stefanie Sarantopoulos
2 The Antibody Molecule
Stefanie Sarantopoulos
3 Antibody-Antigen Interactions
Stefanie Sarantopoulos
4 Antibody Responses
Stefanie Sarantopoulos
5 Selecting the Antigen
Edward A. Greenfield, James DeCaprio, and Mohan Brahmandam
6 Immunizing Animals
Edward A. Greenfield
7 Generating Monoclonal Antibodies
Edward A. Greenfield
8 Growing Hybridomas
Edward A. Greenfield
9 Characterizing Antibodies
Frances Weis-Garcia and Robert H. Carnahan
10 Antibody Purification and Storage
Jordan B. Fishman and Eric A. Berg
11 Engineering Antibodies
James Dasch and Amy Dasch
12 Labeling Antibodies
Eric A. Berg and Jordan B. Fishman
13 Immunoblotting
Larisa Litovchick
14 Immunoprecipiation
James DeCaprio and Thomas O. Kohl
15 Immunoassays
Thomas O. Kohl and Carl A. Ascoli
16 Cell Staining
Scott J. Rodig
17 Antibody Screening using High Throughput Flow Cytometry
Thomas D.l. Duensing and Susan R. Watson
Appendix I: Electrophoresis
Appendix II: Protein Techniques
Appendix III: General Information
Appendix IV: Bacterial Expression
Appendix V: Cautions
Index
More information is available about Antibodies at a preview site for the book: http://antibodiesmanual.org/.

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