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In This Issue June 2010 New Books |
Drosophila Neurobiology: A Laboratory Manual Based on Cold Spring Harbor Laboratory’s long running Neurobiology of Drosophila course, this manual offers practical advice to all researchers interested in using Drosophila as an experimental model for investigating the nervous system.
NF-κB: A Network Hub Controlling Immunity, Inflammation, and Cancer This vital reference covers the structure of NF-κB, its DNA-binding activity and specificity, the role of the inhibitor I-κB, canonical and alternative mechanisms of NF-κB activation, and the use of NF-κB inhibitors in cancer therapy.
Written and edited by experts in the field, this volume provides a comprehensive review of the functions of the p53 family. The contributors examine the normal roles of these transcription factors, the regulatory mechanisms that control p53 activity, and the part played by p53 mutations in tumorigenesis. They also discuss the evolution of the p53 family, which may originally have arisen to protect the integrity of the germ line.
This volume provides a comprehensive account of the environment of the early Earth and describes how the first self-replicating systems emerged from prebiotic chemistry and evolved into primitive cell-like entities.
Evolution:
The Molecular Landscape This book presents the latest advances in research into evolution, with a focus on the molecular bases for evolutionary change.
Career Opportunities in Clinical Drug Research This book is an easy-to-follow handbook that introduces readers to entry-level clinical job opportunities and explains how to qualify for them, with a particular emphasis on how to gain “clinical experience” that a hiring manager will accept.
Originally published in 1981, and edited by Kenneth J. Muller, John G. Nicholls, and Gunther S. Stent, this monograph is still considered a “must read” for graduates in neurobiology. It has been out of print for many years; however, some recent inquiries have prompted us to reprint it and make it available at an affordable price. |
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Support
Research and Did you know that buying directly from CSHL Press enables you to save money on any title we publish? As a member of our Discount Program, you will enjoy prices that are frequently lower than those of any other online site – including Amazon. Regardless of where you make your purchase, all revenue from sales of CSHL Press publications supports research at Cold Spring Harbor Laboratory. However, when you buy directly from our website, you provide a greater level of assistance. As a member of our Discount Program, you will also be the first to hear about new titles arriving in our warehouse and will receive exclusive special offers. For complete details, click here. |
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“This book was published to fill a void in the knowledge of mouse hematopoiesis and hematological cell morphology. The book comes with a poster for easy visual reference as well as a DVD with eight individual chapter referenced movies (shown in QuickTime) to allow the learner to see how to perform the procedures discussed in said chapters. The text is broken down into six chapters which kept me endlessly fascinated from the first page to the last. One of the most attractive things about this book is the language in which it is written. This language allows the reader to learn the medical and laboratory terminology of hematology through written, pictorial and video examples.” —Veterinary Support Personnel Network
“Untangling the Double Helix tells a highly stylized story that is marked by a clarity of thought and expression. Its goal is not to be a comprehensive volume that encompasses every aspect of topoisomerases. Rather, it serves as a well-written, often personal overview of the field. As a result, this engaging publication occupies a unique niche in the world of topoisomerases. It contains enough detail to make it a welcome addition to the bookshelves of investigators in the field, yet it is sufficiently accessible to serve as a superb introduction for those who are not yet acquainted with these fascinating enzymes.” —The Quarterly Review of Biology
“[T]he book is very well presented, written in a logical
and concise way, and unequivocally solid in terms of scientific content,
with many ready-to-use, robust procedures tested and validated over
the years by recognized experts. |
 Even Craig Venter himself, no innocent in the world of publicity, says he is surprised by press reactions to his announcement on May 20 that bacterial cells could be made viable by a chemically synthesized genome. Speaking at a meeting at Cold Spring Harbor Laboratory on May 31, Venter said that the reporting was the strangest he had encountered in his career. Nevertheless, references in interviews and press releases to "synthetic cells" were readily translated into media stories about "new life forms" and President Obama has asked the White House bioethics commission to report back to him on the significance of this development. The science involved is a formidable technical achievement, and, as Venter outlined, the culmination of 15 years' effort. But it raises no new ethical concerns. Instead it resurrects anxieties that have surrounded genetic technologies for decades, many of them thoughtfully and eloquently addressed in a number of recent Cold Spring Harbor books. |
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The articles below were specially selected
by our journal editors and |
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MicroRNA-140
plays dual roles in both cartilage development and homeostasis |
Visit our Sale Shelf where you will find steeply discounted laboratory manuals, textbooks, and monographs! Don’t miss this opportunity to add exceptional books to your professional library at price reductions of 50% or more. Quantities are limited, so don’t delay — stock up now! Here’s just a small sampling of what you will find on the Sale Shelf.
(Sale Shelf discounts apply to direct website purchases by individual US customers only. Please note that all sales are final.) |
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mRNA-seq
with agnostic splice site discovery for nervous system transcriptomics
tested in chronic pain. |
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Prefrontal
cortex lesions and sex differences in fear extinction and perseveration. |
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Translational
repression by PUF proteins in vitro. |
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Cold Spring Harbor Perspectives in Biology in New Scientist In “Self-starter: Life got going all on its own,” New Scientist features Cold Spring Harbor Perspectives author Michael Yarus and his work on RNA and the origin of life. Yarus’ recent article takes us back to a time before the RNA world—when the initial Darwinian ancestor (Ida) appeared. Genes & Development in the Los Angeles Times A blog post at the LA Times, “How a fat cell comes to be,” reports on recent work by Mitchell Lazar and colleagues to understand epigenomic changes that occur during the formation of mature adipocytes. Their research paper was recently published in Genes & Development. Genome Research in the US News & World Report US News & World Report covered a novel strategy of mRNA-sequencing analysis that revealed new insights into the biology of chronic pain. The article describes how researchers studied the transcriptome of neurons in rats and identified candidate genes implicated in pain response. |
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• Spanning the complete spectrum of the molecular life sciences Read these essential papers in this month’s issue: Pierre
Vanderhaeghen reviews axon pruning and neuronal cell death Giuseppe
Biamonti and Claire Vourc’h discuss nuclear stress bodies Drug
design — Andreas Joerger and Alan Fersht on targeting p53 Follow Cold Spring Harbor Perspectives on Twitter: http://twitter.com/cshperspectives Visit here for this month’s complete table of contents and to learn more. Read the complete launch press release here. |
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First web availability of the renowned book series In the late fifties, Tim Loeb, a new graduate student at The Rockefeller University, came into my laboratory and asked whether I thought it was possible that there were male-specific bacteriophages for E. coli. At that time I had just succeeded in transferring the E. coli F factor into Salmonella and noted that these Salmonella F+ had become resistant to a Salmonella phage, SP5. I therefore quickly responded in the affirmative and off he went to a raw sewage plant in New York City. Using a set of otherwise isogenic male and female strains of E. coli, K-12, the “F” series of phages were quickly isolated. F1, the first isolate, which later was shown to be a filamentous DNA-containing phage, was set aside because it made relatively poor plaques, and f2, the second isolate, was chosen for further study. Little did we think at the time that a whole new area of study was in the offing; that from this phage and its relatives one would obtain insights into the details of the mating organelle in E. coli, the elements involved in protein biosynthesis and verification of the fidelity of in vitro protein synthesis, the nature of initiation and termination signals on messenger RNA, the first nucleic acid to be replicated faithfully in vitro, a tool for testing the fidelity of nucleotide sequencing eventually leading to the first gene to be completely sequenced, and much more. Read More. The CSH Monograph Archive offers the complete collection of scholarly monographs published by Cold Spring Harbor Laboratory Press from 1970 to 2009. The archive’s 59 full-text volumes provide the life science community with definitive reviews of progress in areas of molecular, cell, and developmental biology, genetics, evolutionary biology, neuroscience, cancer biology, and molecular pathology. Each text is written and commissioned by foremost researchers in their particular discipline. It is available online as a complete collection for one-time purchase (with perpetual access) or on pay-per-view basis by book chapter.
Cold Spring Harbor Monograph Archive |
At the Helm: Leading Your Laboratory, Second Edition At the Bench: A Laboratory Navigator, Updated Edition Lab Dynamics: Management Skills for Scientists Statistics at the Bench: A Step-by-Step Handbook for Biologists DNA Science: A First Course, Second Edition Live Cell Imaging: A Laboratory Manual, Second Edition |
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Gel electrophoresis is one of the most important and frequently
used techniques in RNA analysis. Electrophoresis is used for RNA
detection, quantification, purification by size and quality assessment.
Gels are involved in a wide variety of methods including northern
blotting, primer extension, footprinting and analyzing processing
reactions. The two most common types of gels are polyacrylamide
and agarose. Polyacrylamide gels are used in most applications
and are appropriate for RNAs smaller than approximately 600 nucleotides
(agarose gels are preferred for larger RNAs). Polyacrylamide
Gel Electrophoresis of RNA describes how to prepare, load and run polyacrylamide
gels for RNA analysis. The article is featured in the June
issue of Cold Spring Harbor Protocols and is freely
available on the journal’s
website. It is part of a suite of basic RNA protocols included
in this month’s
issue that provide an early preview of the forthcoming RNA:
A Laboratory Manual due later this year from Cold Spring Harbor Laboratory Press. To enjoy access to the entire collection of methods in Cold Spring Harbor Protocols, subscribe today! Free trials are available. Ask your librarian to request a trial for your institution or use our convenient online form. Submit to Cold Spring Harbor Protocols! Cold Spring Harbor Protocols also welcomes your new and improved protocols — click here to view our online manuscript submission instructions. |
The dynamic nature of biological processes has long been difficult to document, as researchers have been limited to static studies based on fixed specimens. Methods like immunocytochemistry or in situ hybridization can only provide accurate information on one organism at one particular time point. As Scott Fraser has remarked, it’s akin to trying to figure out the rules of football from looking at a set of still photographs taken during a game. But recent developments in imaging techniques, particularly the use of Green Fluorescent Protein (GFP) and its variants, have provided nondestructive ways to study dynamic processes over time, taking our understanding into the fourth dimension. These new imaging techniques generate an enormous amount of digital image data, which can be difficult to cope with as it builds up over time. Computer-based image analysis is required for the extraction of reproducible and quantitative information. Previously, Cold Spring Harbor Protocols has featured Khuloud Jaqaman and Gaudenz Danuser’s case study using particle tracking to study cellular dynamics. In the June issue of the journal, Roland Eils and colleagues present Tracking and Quantitative Analysis of Dynamic Movements of Cells and Particles. The article sketches a general workflow for quantitative analysis of live cell images and details automated methods for image analysis including preprocessing, segmentation, registration, tracking and classification. |
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Cold Spring Harbor Laboratory is a private, nonprofit institution for research and education in the biological sciences. The Lab is internationally recognized for its excellence in research in cancer, neurobiology, plant genetics, and bioinformatics, and offers a broad range of educational programs for professional scientists and scientists-in-training, from elementary school students to Nobel laureates. Check the job listings below for opportunities to become a part of this important institution. Here are a few that are currently listed:
Check out the latest job opportunities at Cold Spring Harbor Laboratory HERE. |
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The Dolan DNA Learning Center (DNALC) is the world’s first science center devoted entirely to genetics education. |
2010 CSHL Meetings & Conferences
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For all other inquiries, please contact: Geraldine Jaitin |
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