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RNAi: A Guide to Gene Silencing

Subject Area(s):  Developmental BiologyCell BiologyMolecular BiologyGeneticsNeurobiology

Edited by Gregory J. Hannon, Cold Spring Harbor Laboratory

© 2003 • 436 pp., illus., index
Printed hardcover •
ISBN  978-087969641-2

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The ability of double stranded RNA to inhibit the expression of genes with an appropriate sequence can be harnessed to silence target genes in vitro in a new and powerful way. This volume combines reliable RNAi protocols for a variety of species with discussion of strategies for the effective design of experiments using this important new technique that is changing the way experimental science is done.


Chapter 1. Sense Cosuppression in Plants: Past, Present, and Future
Richard A. Jorgensen
Chapter 2. Transgene Cosuppression in Animals
James A. Birchler, Manika Pal–Bhadra, and Utpal Bhadra
Chapter 3. Regulation of the Genome by Double–stranded RNA
Marjori Matzke, M. Florian Mette, Tatsuo Kanno, Werner Aufsatz, and Antonius J.M. Matzke
Chapter 4. RNAi in Caenorhabditis elegans
René F. Ketting, Marcel Tijsterman, and Ronald H.A. Plasterk
Chapter 5. Small RNAs, Big Biology: Biochemical Studies of RNA Interference
Gregory J. Hannon and Phillip D. Zamore
Chapter 6.shRNA-mediated Silencing of Mammalian Gene Expression
Michael T. McManus and Douglas S. Conklin
Chapter 7. Transposable Elements, RNA Interference, and the Origin of Heterochromatin
Rob Martienssen, Tom Volpe, Zach Lippman, Ann-Valerie Gendrel, Catherine Kidner, Pablo Rabinowicz, and Vincent Colot
Chapter 8. The Ribonuclease III Superfamily: Forms and Functions in RNA Maturation, Decay, and Gene Silencing
Allen W. Nicholson
Chapter 9. RNA–dependent RNA Polymerase in Gene Silencing
Luyun Huang, John Gledhill, and Craig E. Cameron
Chapter 10. Structure and Function of Heterochromatin: Implications for Epigenetic Gene Silencing and Genome Organization
Ira M. Hall and Shiv I.S. Grewal
Chapter 11. Total Interference: Genome–wide RNAi Screens in C. elegans
Andrew G. Fraser and Julie Ahringer
Chapter 12. PTGS Approaches to Large Scale Functional Genomics in Plants
Tessa M. Burch–Smith, Jennifer L. Miller, and S.P. Dinesh–Kumar
Chapter 13. Mammalian RNA Interference
Thomas Tuschl
Chapter 14. RNAi in Avian Embryos
Esther T. Stoeckli
Chapter 15.
Guide to RNAi in Mouse Oocytes and Preimplantation Embryos
Paula Stein and Petr Svoboda
Chapter 16. RNAi Technologies in Drosophila Cell Culture
Scott M. Hammond
Chapter 17. RNAi Applications in Drosophila melanogaster
Richard W. Carthew
Chapter 18. RNA Interference in Trypanosoma brucei and Other Nonclassical Model Organisms
Elisabetta Ullu and Christian Tschudi


review:  “Because of the overwhelming plethora of studies, this book, written by leaders in the field, arrives as a welcome road map for beginners and experts alike. The volume provides a broad overview of the basic mechanisms and applications of RNAi to different model organisms for functional gene analysis, including nonclassical model systems such as the parasitic protozoan Trypanosoma brucei and the chicken embryo. In addition, several chapters are devoted to subjects that are more loosely related to RNAi and thus less frequently reviewed in papers in the field....RNAi is indeed obligatory reading for anyone interested in modern molecular biology.”

review:  “This book provides a useful and relatively comprehensive summary of progress in the field, and it is likely that every biologist will find something of interest. While experts in this field will be able to update their knowledge on RNAi and its applications (for example, its use in high–throughput screening), the book should also serve as a useful introduction to the world of small RNAs for the non–expert. An added bonus is the inclusion of many protocols that explain the details of key RNAi experiments that once again should prove useful to the expert and non–expert alike.”
      —Nature Cell Biology