Ion Channels: A Laboratory Manual

Subject Area(s): Biochemistry; Biophysics; Neurobiology

Edited by Paul J. Kammermeier, University of Rochester Medical Center; Ian Duguid, Centre for Integrative Physiology, University of Edinburgh; Stephan Brenowitz, Janelia Research Campus, Howard Hughes Medical Institute

Download a Free Excerpt from Ion Channels: A Laboratory Manual:

Index
Voltage-Gated Na+ Channels: Not Just for Conduction
Sample Protocol: Combining Optogenetics and Electrophysiology to Analyze Projection Neuron Circuits

© 2017 • 200 pages, illustrated (28 color and 8 B&W), index
Paperback • ~~$90~~ 72.00
ISBN 978-1-621821-21-2
You save: 20%
You will receive free shipping on this item at checkout.
Free shipping offer applies to direct website purchases by individual U.S. and Canada customers only.

This title also available in: Hardcover

Print Book + eBook
Best value! ~~$171.9~~ $81.00

Print Book ~~$90~~ $72.00

eBook ~~$81.90~~ $61.42

Bulk discounts available for your lab or class. Click here to inquire.

eBooks use Adobe Digital Editions software. Click here for more information.

Description
Contents
Reviews
Related Titles

Description

The propagation of information through the nervous system depends on rapid changes in the electrical potential across excitable cell membranes and the generation of action potentials—the fundamental unit of neuronal communication. Changes in membrane potential occur when ion channels—pore-forming membrane proteins—open to facilitate the passage of specific ions (e.g., Na⁺, K⁺) across the cell membrane in response to a stimulus. Defects in ion channels can lead to a wide range of diseases of the nervous system.

This laboratory manual provides state-of-the-art techniques for investigating ion channel properties and function, particularly in the nervous system. Contributors present electrophysiological methods to examine single-channel activity in cultured cells, to study synaptic plasticity and circuit dynamics in brain slice preparations, and to perform whole-cell recordings in awake and freely moving animals. The use of optogenetic tools to study cellular and circuit dynamics in organotypic slice cultures is also covered. Many of the protocols can be adapted for other ion channels, cell types, and experimental preparations.

The manual includes background on the structure, function, and regulation of different voltage- and ligand-gated ion channels. Therefore, it is a useful resource for all cell biologists and neuroscientists seeking to further understand the complex roles of ion channels in physiology and disease.

CHAPTER 1 Ion Channels: History, Diversity, and Impact
INTRODUCTION
Ion Channels: History, Diversity, and Impact: Stephan Brenowitz, Ian Duguid, and Paul J. Kammermeier
CHAPTER 2 Voltage-Gated Potassium Channels: A Structural Examination of Selectivity and Gating
INTRODUCTION
Voltage-Gated Potassium Channels: A Structural Examination of Selectivity and Gating: Dorothy M. Kim and Crina M. Nimigean
CHAPTER 3 Voltage-Gated Na⁺ Channels: Not Just for Conduction
INTRODUCTION
Voltage-Gated Na⁺ Channels: Not Just for Conduction: Larisa C. Kruger and Lori L. Isom
CHAPTER 4 Measuring Ca²⁺-Dependent Modulation of Voltage-Gated Ca²⁺ Channels in HEK-293T Cells
PROTOCOL
1 Measuring Ca²⁺-Dependent Modulation of Voltage-Gated Ca²⁺ Channels in HEK-293T Cells: Jessica R. Thomas and Amy Lee
CHAPTER 5 Strategies for Investigating G-Protein Modulation of Voltage-Gated Ca²⁺ Channels
INTRODUCTION
Strategies for Investigating G-Protein Modulation of Voltage-Gated Ca²⁺ Channels: Van B. Lu and Stephen R. Ikeda
PROTOCOL
1 G-Protein Modulation of Voltage-Gated Ca²⁺ Channels from Isolated Adult Rat Superior Cervical Ganglion Neurons: Van B. Lu and Stephen R. Ikeda
CHAPTER 6 Hyperpolarization-Activated Cyclic Nucleotide-Gated Channel Currents in Neurons
INTRODUCTION
Hyperpolarization-Activated Cyclic Nucleotide-Gated Channel Currents in Neurons: Mala M. Shah
PROTOCOL
1 Recording Hyperpolarization-Activated Cyclic Nucleotide-Gated Channel Currents (/h) in Neurons: Mala M. Shah
CHAPTER 7 Single-Channel Recording of Ligand-Gated Ion Channels
INTRODUCTION
Single-Channel Recording of Ligand-Gated Ion Channels: Andrew J.R. Plested
PROTOCOL
1 Single-Channel Recording of Glycine Receptors in Human Embryonic Kidney (HEK) Cells: Andrew J.R. Plested and Jelena Baranovic
CHAPTER 8 Measuring the Basic Physiological Properties of Synapses
INTRODUCTION
Measuring the Basic Physiological Properties of Synapses: Matthew A. Xu-Friedman
PROTOCOL
1 Preparing Brain Slices to Study Basic Synaptic Properties: Matthew A. Xu-Friedman
CHAPTER 9 Cellular and Synaptic Properties of Local Inhibitory Circuits
INTRODUCTION
Cellular and Synaptic Properties of Local Inhibitory Circuits: Court Hull
PROTOCOL
1 Measuring Feedforward Inhibition and Its Impact on Local Circuit Function: Court Hull
CHAPTER 10 Fast Cholinergic Synaptic Transmission in the Mammalian Central Nervous System
INTRODUCTION
Fast Cholinergic Synaptic Transmission in the Mammalian Central Nervous System: Michael Beierlein
PROTOCOL
1 Examining Cholinergic Synaptic Signaling in the Thalamic Reticular Nucleus: Rajan Dasgupta, Frederik Seibt, Yan-Gang Sun, and Michael Beierlein
CHAPTER 11 In Vitro Investigation of Synaptic Plasticity
INTRODUCTION
In Vitro Investigation of Synaptic Plasticity: Therese Abrahamsson, Txomin Lalanne, Alanna J. Watt, and P. Jesper Sjöström
PROTOCOLS
1 Long-Term Potentiation by Theta-Burst Stimulation Using Extracellular Field Potential Recordings in Acute Hippocampal Slices: Therese Abrahamsson, Txomin Lalanne, Alanna J. Watt, and P. Jesper Sjöström
2 Using Multiple Whole-Cell Recordings to Study Spike-Timing-Dependent Plasticity in Acute Neocortical Slices: Txomin Lalanne, Therese Abrahamsson, and P. Jesper Sjöström
CHAPTER 12 Stimulating Neurons with Heterologously Expressed Light-Gated Ion Channels
INTRODUCTION
Stimulating Neurons with Heterologously Expressed Light-Gated Ion Channels: J. Simon Wiegert, Christine E. Gee, and Thomas G. Oertner
PROTOCOLS
1 Preparation of Slice Cultures from Rodent Hippocampus: Christine E. Gee, Iris Ohmert, J. Simon Wiegert, and Thomas G. Oertner
2 Viral Vector-Based Transduction of Slice Cultures: J. Simon Wiegert, Christine E. Gee, and Thomas G. Oertner
3 Single-Cell Electroporation of Neurons: J. Simon Wiegert, Christine E. Gee, and Thomas G. Oertner
CHAPTER 13 Combining Optogenetics and Electrophysiology to Analyze Projection Neuron Circuits
PROTOCOL
1 Combining Optogenetics and Electrophysiology to Analyze Projection Neuron Circuits: Naoki Yamawaki, Benjamin A. Suter, Ian R. Wickersham, and Gordon M.G. Shepherd
CHAPTER 14 Whole-Cell Recording in the Awake Brain
INTRODUCTION
Whole-Cell Recording in the Awake Brain: Doyun Lee and Albert K. Lee
PROTOCOLS
1 In Vivo Patch-Clamp Recording in Awake Head-Fixed Rodents: Doyun Lee and Albert K. Lee
2 Efficient Method for Whole-Cell Recording in Freely Moving Rodents Using Ultraviolet-Cured Collar-Based Pipette Stabilization: Doyun Lee and Albert K. Lee
APPENDIX General Safety and Hazardous Material Information
INDEX

Reviews

“The book...is an extremely useful guide for the study of ion channels. This volume is well written and describes in appropriate detail protocols to study diverse ion channel types in various contexts.... The protocols, including troubleshooting guides, will be an invaluable resource for initiating techniques in a research laboratory. The manual would also be a fantastic basis for a laboratory class for advanced undergraduates or graduate students.”
—The Quarterly Review of Biology

Related Titles

Ion Channels: A Laboratory Manual [Hardcover]

Ion Channels: A Laboratory Manual

Description

Contents

Reviews

Related Titles