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Genes & Development

Edited By Laureen Connell, Cold Spring Harbor Laboratory Press

Online Features: Genes and Development Online  – Internet edition

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© 2024 • ISSN 0890-9369; Volume 38 includes full online access
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January - December 2024, 24 monthly issues
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Description

Genes & Development publishes high-quality research papers of broad general interest and biological significance in the areas of molecular biology, molecular genetics, and related fields. G&D publishes two research formats; Research papers and short Research Communications, in addition to Review Articles and Perspectives. G&D consistently ranks in the top 10 among primary research journals in all fields of science.
This journal is published in association with The Genetics Society.
Subscription to the print journal includes full access to the online edition (online-only subscriptions are available upon request).

 
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Genome Research

Edited By Hillary Sussman, Cold Spring Harbor Laboratory Press; Aravinda Chakravarti, NYU Langone Health; Richard Gibbs, Baylor College of Medicine; Eric Green, Bethesda, MD; Richard Myers, HudsonAlpha Institute for Biotechnology; Evan Eichler, University of Washington; Anne Ferguson-Smith, University of Cambridge; Kateryna Makova, Penn State University

Online Features: Internet edition – Genome Research Online at Stanford University Libraries' HighWire Press; Electronic supplementary material – auxiliary data available only via the journal's online edition

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© 2024 • ISSN: 1088-9051; Volume 34 includes full online access
FREE TRIAL ACCESS offer for libraries! Contact [email protected].
January - December 2024, 12 monthly issues
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Description

In August 1995, Cold Spring Harbor Laboratory Press began publication of a new, monthly, international, peer-reviewed journal, Genome Research. The journal focuses on genome studies in all species, and presents research that provides or aids in genome-based analyses of biological processes. The journal represents a nexus point where genomic information, applications, and technology come together with biological information to create a more global understanding of all biological systems.

Among the research considered by the journal are novel reports on gene discovery, comparative genome analyses, evolution studies, informatics, genome structure and function, technological innovations and applications, statistical and mathematical methods, cutting-edge genetic and physical mapping and DNA sequencing, and other reports that present data where sequence information is used to address biological concerns.

New data in these areas are published as research papers in the form of articles and letters, or methods and resource reports that provide novel information on methodologies or resources that will be of interest to a broad readership. Complete data sets are presented electronically on the journal's web site where appropriate. The journal also provides review articles, perspectives, and Insight/Outlook articles, which presents commentary on the latest advances published both here and elsewhere, placing such progress in its broader biological context.

Subscription to the print journal includes full access to the online edition (online-only subscriptions are not available).

 
 

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Learning & Memory

Edited By John H. Byrne, University of Texas School of Medicine

Online Features: Internet edition – Learning & Memory Online at Stanford University Libraries' HighWire Press

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© 2024 • ISSN 1549-5485; Volume 31; includes full online access
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January - December 2024, 12 online  issues
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Learning & Memory is the journal of choice for interdisciplinary studies on synaptic plasticity, learning, and memory. Until now, there has been no one journal devoted to this area of study and no dominant journal that demands attention by serious workers in the area, regardless of specialty. Spanning molecules, cells, systems, and behavior, L&M considers research using approaches from cellular and molecular biology, genetics, biochemistry, computational neuroscience, neurophysiology, neuropharmacology, neuroanatomy, behavior, and cognition. Learning & Memory provides a forum for these investigations in the form of original research papers, review articles, and brief communications.

 
 

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CSH Perspectives in Medicine

Online Features: CSH Perspectives in Medicine – CSH Perspectives in Medicine at Stanford University Libraries' HighWire Press

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© 2024 • ISSN: 2157-1422; Volume 14 includes full online access
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[email protected].

January - December 2024, 12 monthly issues
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Cold Spring Harbor Perspectives in Medicine is a new online publication covering everything from the molecular and cellular bases of disease to translational medicine and new therapeutic strategies. Each issue offers reviews on different aspects of a variety of diseases and the tissues they affect. The contributions are written by experts in each field and commissioned as Subject Collections by a board of eminent scientists and physicians, all of whom are acknowledged to be key figures in their particular fields. These Subject Collections gradually accumulate articles as new issues of the journal are published and, when complete, each represents a comprehensive survey of the field it covers. Cold Spring Harbor Perspectives in Medicine is thus unmatched in its depth of coverage and represents an essential source where readers can find informed surveys and critical discussion of advances in molecular medicine.

Please visit www.perspectivesinmedicine.org for complete information about the journal.

Subject Coverage includes:

  • Addiction
  • Aging
  • Alzheimer Disease
  • Anemia
  • Antibiotic Resistance
  • Bacterial Pathogens
  • Bone Disease
  • Cancer
  • Cystic Fibrosis
  • Diabetes
  • Heart Disease
  • Hemoglobin
  • HIV
  • Influenza
  • Malaria
  • Multiple Sclerosis
  • Muscular Dystrophy
  • Parkinson's Disease
  • Prion Diseases
  • Skin Diseases
  • Schizophrenia
 
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CSH Perspectives in Biology

Online Features: CSH Perspectives in Biology – CSH Perspectives in Biology at Stanford University Libraries' HighWire Press

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© 2024 • ISSN: 1943-0264; Volume 16 includes full online access
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[email protected].

January - December 2024, 12 monthly issues
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A new online publication for the molecular life sciences

Cold Spring Harbor Perspectives in Biology provides the scientific community with comprehensive, systematic surveys of topics in the molecular life sciences where research is breaking new ground

In July 2009, Cold Spring Harbor Laboratory Press launched a new monthly journal, Cold Spring Harbor Perspectives in Biology, that provides comprehensive, systematically structured surveys of research in exciting areas of molecular and cellular biology, genetics, developmental biology, neuroscience, cancer biology, and molecular pathology.

For decades, Cold Spring Harbor Laboratory has complemented its groundbreaking research with publication of a variety of highly prestigious titles written and edited by leading scientists. These include the journals Genes & Development and Genome Research, the bible of research methods, Molecular Cloning, online teaching resources, and numerous other laboratory manuals, monographs, handbooks, journals, and textbooks.

Cold Spring Harbor Perspectives in Biology (http://www.cshperspectives.org; ISSN 1943-0264) is the latest, and perhaps most ambitious, addition to this stable. Its depth of coverage is unmatched, spanning the entire spectrum of the molecular life sciences and therefore providing an essential resource for all areas of bioscience research.

According to the journal's Executive Editor, Richard Sever, who has worked on traditional review journals including the Current Opinion and Trends titles, Cold Spring Harbor Perspectives in Biology has a new and unique approach. "There are many review articles published each year, but they're usually on one particular aspect of one particular field," he says. "CSH Perspectives is different. We cover subjects by asking experts to consider how best to structure a comprehensive survey of their field, and then commission articles from all the key figures in that field. And we cover everything in bioscience from Alzheimer's to plant biology."

CSH Perspectives articles are all commissioned as �collections� by a board of eminent academic editors and gradually accumulate online at the journal's website each month as they are accepted and published. The debut issue features articles commissioned by Elaine Fuchs, Michael Karin, W. James Nelson, and Peter Lawrence and includes contributions from Nobel laureate David Baltimore, molecular motor expert Nobutaka Hirokawa, and developmental biologist Jim Smith. These set the stage for subject collections on symmetry breaking, morphogen gradients, cell junctions, and the immune signaling molecule NF-kB. Next in the pipeline are articles on the origin of life, the p53-family of tumor suppressors, the nucleus, and immune tolerance.

The new publication is produced online in partnership with Stanford University Library's HighWire Press, taking advantage of their new H20 platform. The state-of-the-art website includes HTML and PDF views, high-resolution artwork, and video material, along with a host of novel features such as author updates, commenting options, and Twitter-powered news feeds.

 
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CSH Protocols

Edited By Alejandro Montenegro-Montero, Cold Spring Harbor Laboratory Press

Online Features: Internet edition – Cold Spring Harbor Protocols at Stanford University Libraries' HighWire Press

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© 2024 • ISSN: 1559-6095; Volume 19; includes full online access
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January - December 2024, 12 monthly issues
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  • The online source of trusted techniques in molecular and cellular biology
  • Contains new and classic protocols presented step-by-step with recipes and troubleshooting
  • Frequently updated and annotated
  • Interactive, customizable, and fully searchable

The development of CSH Protocols is a response to requests from scientists for authoritative information about techniques with a broad editorial scope, delivered through a state-of-the-art online interface. At this site, you will find methods from Molecular Cloning along with selected protocols from many of our best-selling manuals, such as Cells and Antibodies, as well as protocols from Cold Spring Harbor's renowned on-site courses. In addition, you will discover new cutting-edge protocols submitted by and commissioned from laboratories worldwide. A peer-reviewed online journal, CSH Protocols is updated monthly.

Features include:
  • Step-by-step, uniformly structured formats for ease of use and printing, with clearly linked materials, cautions, recipes, and troubleshooting
  • Customizable, topic-based e-mail alerts for the latest updates in a particular field
  • Personal folders for storing favorite protocols and searches
  • Navigation tools including a specifically designed taxonomy for browsing and a variety of full-text search options
  • A kit search tool for fast access to time-saving resources
  • An online manuscript submission system for authors to contribute new protocols

CSH Protocols — More Than Just Methods.

Visit www.cshprotocols.org for more details on features, subscriptions, and manuscript submission.

  • Available exclusively via institutional site license
  • ISSN 1559-6095 / online, monthly

Read within Top Institutions and Companies Such as:

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RNA

Edited By Timothy W. Nilsen, Editor-in-Chief, Case Western Reserve University; Thomas R. Cech, Howard Hughes Medical Institute; David Engelke, University of Michigan; Eric Westhof, CNRS Strasbourg; Marvin Wickens, University of Wisconsin

Online Features: Internet edition – RNA Online at Stanford University Libraries' HighWire Press

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© 2024 • ISSN 1355-8382; Volume 30; includes full online access.
FREE TRIAL ACCESS offer for libraries! Contact [email protected].
(Published by CSHL Press for The RNA Society)
January - December 2024, 12 monthly issues
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RNA, the publication of the RNA Society, publishes high quality research papers that provide significant new insight into any area of RNA biology, including molecular biology, cell biology, biochemistry, bioinformatics, genetics and evolution. RNA publishes two research formats, articles and short reports. In addition, the journal publishes novel methodologies, reviews and perspectives. RNA provides a unique resource to all members of the RNA community and those that wish to keep abreast of this quickly moving area.

 
 

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The Biology of Lipids: Trafficking, Regulation, and Function, Second Edition


Subject Area(s):  Cell BiologyBiochemistryPlant Biology

Edited by Robert G. Parton, The University of Queensland; Kai Simons, Max-Planck-Institute of Molecular Cell Biology and Genetics and Lipotype GmbH

Due April 2024 • 300 pages (approx.), illustrated, index
Hardcover •
ISBN  978-1-621824-74-9

  •     Description    
  •     Contents    

Description

Lipids are the major component of cell and organelle membranes. They are essential for maintaining cell integrity and defining distinct subcellular compartments but also play important roles in a variety of other cellular processes. Membrane fission and fusion and movement of lipid-enveloped vesicles underlie the majority of protein trafficking in cells and are critical for the function of synapses in the nervous system. In addition, lipids participate in numerous signaling pathways that connect cell-surface receptors with intracellular effectors.

Written and edited by experts in the field, this new edition of the highly successful Cold Spring Harbor volume on lipids provides a comprehensive update on lipid cell biology. The contributors discuss the organization of lipids in cellular membranes, membrane biophysics, and the phase transitions lipids undergo. They examine tools for analysis of lipids and lipid modifications. They also review our understanding of lipid sorting and its role in maintenance of organelle identity.

The volume covers the functions of lipids in various signal transduction pathways, as well as the roles of cholesterol, extracellular vesicles, and the importance of lipids in the unfolded protein response. Including discussion of the importance of lipids for synaptic activity and memory, it is an indispensable reference not only for cell biologists and biochemists but neurobiologists and any other researchers interested in the importance of lipids in cell physiology.

Contents

Seeing the Membrane from Both Sides Now: Lipid Asymmetry and Its Strange Consequences
Milka Doktorova, Ilya Levental, and Frederick A. Heberle
A Survey of Models of Cell Membranes: Towards a New Understanding of Membrane Organization
Satyajit Mayor, Abrar Bhat, and Akihiro Kusumi
The Membrane Phase Transition Gives Rise to Responsive Plasma Membrane Structure and Function
Sarah A. Shelby and Sarah L. Veatch
Lipid Sorting and Organelle Identity
Yeongho Kim and Christopher G. Burd
Endocytic Roles of Glycans on Proteins and Lipids
Ludger Johannes, Massiullah Shafaq-Zadah, Estelle Dransart, Christian Wunder, and Hakon Leffler
ER Membrane Homeostasis and the Unfolded Protein Response
Robert Ernst, Mike F. Renne, Aamna Jain, and Alexander von der Malsburg
Intracellular Cholesterol Trafficking
Elina Ikonen and Vesa M. Olkkonen
Phospholipase Modulation of Synaptic Membrane Landscape: Driving Force Behind Memory Formation?
Tristan P. Wallis and Frédéric A. Meunier
Phosphoinositides in New Spaces
Elizabeth Michele Davies, Christina Anne Mitchell, and Harald Alfred Stenmark
Chemical Approaches for Measuring and Manipulating Lipids at the Organelle Level
Masaaki Uematsu and Jeremy M. Baskin
Beyond Fluidity: The Role of Lipid Unsaturation in Membrane Function
Takeshi Harayama and Bruno Antonny
The Role of Membrane Lipids in the Formation and Function of Caveolae
Anne K. Kenworthy, Bing Han, Nicholas Ariotti, and Robert G. Parton
RAS GTPases and Interleaflet Coupling in the Plasma Membrane
Junchen Liu, Neha Arora, and Yong Zhou
Lipids in Extracellular Vesicles: What Can Be Learnt About Membrane Structure and Function?
Tore Skotland, Alicia Llorente, and Kirsten Sandvig
Membrane Epilipidome – Lipid Modifications, Their Dynamics and Functional Significance
Sider Penkov and Maria Fedorova
Cholesterol, Eukaryotic Lipid Domains, and an Evolutionary Perspective of Transmembrane Signaling
Yan Shi, Hefei Ruan, Yanni Xu, and Chunlin Zo
How Neuromembrane Lipids Modulate Membrane Proteins: Insights from G Protein-Coupled Receptors (GPCRs) and Receptor Tyrosine Kinases (RTKs)
Mykhailo Girych, Waldemar Kulig, Giray Enkavi, and Ilpo Vattulainen
Index
   

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Mosquitoes: A Laboratory Manual


Subject Area(s):  BiotechnologyLaboratory Techniques

Edited by Laura B. Duvall, Columbia University; Benjamin J. Matthews, The University of British Columbia

Download a Free Excerpt from Mosquitoes: A Laboratory Manual:

Preface
Methods to Assess Blood and Nectar Meals in Aedes aegypti Mosquitoes
Index


Due May 2024 • 771 pages, illustrated (111 color and 7 B&W), index
Paperback •
ISBN  978-1-621824-41-1

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  •     Description    
  •     Contents    

Description

Mosquitoes have been studied for centuries by entomologists, epidemiologists, biologists, geneticists, and ecologists. Early investigations primarily focused on their anatomy, life cycles, and feeding habits. As scientific knowledge progressed, however, the research scope expanded and so has our understanding of the underlying biology of mosquito genetics, physiology, behavior, and pathogen transmission dynamics.

This laboratory manual provides a comprehensive collection of experimental procedures for research using mosquitoes. It includes basic methods for mosquito rearing, egg collection, scoring mating status, scoring reproductive and host-seeking behaviors, imaging RNA and proteins, and evaluating physiological responses to stimuli. The collection also features protocols for genetic manipulation of mosquitoes using RNAi and CRISPR–Cas9 genome editing. Methods to evaluate insecticide resistance are also described.

The methods in this manual focus primarily on Aedes and Anopheles mosquitoes, although we also provide rearing protocols for Culex, and many of the protocols included may also work well in multiple species, with minor modifications. This manual aims to serve both as a critical reference for laboratories already working with mosquitoes and as a comprehensive guide for those starting to use mosquitoes in their experimental work.

Contents

Preface
SECTION 1. REARING AND HUSBANDRY
CHAPTER 1
INTRODUCTION
Overview of Aedes aegypti and Use in Laboratory Studies
Margot P. Wohl and Conor J. McMeniman
PROTOCOLS
1 Batch Rearing Aedes aegypti
Margot P. Wohl and Conor J. McMeniman
2 Single-Pair and Small-Group Crosses of Aedes aegypti
Margot P. Wohl and Conor J. McMeniman
CHAPTER 2 INTRODUCTION
Considerations for Rearing and Maintaining Anopheles in the Laboratory
Laura N. Leite, Priscila Bascuñán, Ellen M. Dotson, and Mark Q. Benedict
PROTOCOLS
1 Anopheles Egg Collection, Disinfection, and Hatching
Laura N. Leite, Priscila Bascuñán, Ellen M. Dotson, and Mark Q. Benedict
2 Anopheles Larval Rearing
Laura N. Leite, Priscila Bascuñán, Ellen M. Dotson, and Mark Q. Benedict
3 Anopheles Adult Anesthesia, Feeding, and Sex Separation
Laura N. Leite, Priscila Bascuñán, Ellen M. Dotson, and Mark Q. Benedict
4 Anopheles Pupa Collection and Sex Identification
Laura N. Leite, Priscila Bascuñán, Ellen M. Dotson, and Mark Q. Benedict
CHAPTER 3 INTRODUCTION
Points to Consider When Establishing and Rearing Culex Mosquitoes in the Laboratory
Megan E. Meuti, Alden Siperstein, and Matthew Wolkoff
PROTOCOLS
1 Establishing a Culex Colony from Field-Collected Eggs
Megan E. Meuti, Alden Siperstein, and Matthew Wolkoff
2 Rearing and Maintaining a Culex Colony in the Laboratory
Megan E. Meuti, Alden Siperstein, and Matthew Wolkoff
CHAPTER 4 INTRODUCTION
Establishing Colonies from Field-Collected Mosquitoes: Special Accommodations for Wild Strains
Noah H. Rose, John J. Shepard, and Diego Ayala
PROTOCOLS
1 Collecting, Storing, and Hatching Aedes aegypti Eggs
Noah H. Rose, John J. Shepard, and Diego Ayala
2 Mosquito Larvae and Pupae Transport from the Field
Diego Ayala, John J. Shepard, and Noah H. Rose
SECTION 2. FEEDING ANALYSIS
CHAPTER 5 INTRODUCTION
Membrane Feeding Devices to Blood Feed Mosquitoes in the Laboratory
Andre Luis Costa-da-Silva
PROTOCOL
1 Artificial Membrane Feeding Mosquitoes in the Laboratory with Glytube
Andre Luis Costa-da-Silva
CHAPTER 6 INTRODUCTION
Trypsin, the Major Proteolytic Enzyme for Blood Digestion in the Mosquito Midgut
Andrew B. Nuss and Monika Gulia-Nuss
PROTOCOL
1 Analyzing Blood Digestion: Estimation of Active Trypsin Levels in the Mosquito Midgut
Jeremiah Reyes, Andrew Nuss, and Monika Gulia-Nuss
CHAPTER 7 INTRODUCTION
Methods to Assess Blood and Nectar Meals in Aedes aegypti Mosquitoes
Krithika Venkataraman, Veronica Jové, and Laura B. Duvall
PROTOCOL
1 Size Quantification of Blood and Sugar Meals in Aedes aegypti Mosquitoes
Krithika Venkataraman, Veronica Jové, and Laura B. Duvall
CHAPTER 8 INTRODUCTION
Characterizing Mosquito Biting Behavior at High Resolution
Gregory P.D. Murray, Emilie Giraud, and Felix J.H. Hol
PROTOCOL
1 Characterizing Mosquito Biting Behavior Using the BiteOscope
Gregory P.D. Murray, Emilie Giraud, and Felix J.H. Hol
CHAPTER 9 INTRODUCTION
Determination of Mosquito Probing and Feeding Time to Evaluate Mosquito Blood Feeding
Ines Martin-Martin, Adeline E. Williams, and Eric Calvo
PROTOCOLS
1 Evaluation of Aedes aegypti Feeding Status
Ines Martin-Martin, Adeline E. Williams, and Eric Calvo
2 Evaluation of Aedes aegypti Penetration, Probing, and Feeding Times on Mice
Ines Martin-Martin, Adeline E. Williams, and Eric Calvo
CHAPTER 10 INTRODUCTION
Mosquito Pollination and Sugar Detection Methods: An Overview
Daniel A.H. Peach
PROTOCOLS
1 Sugar Detection in Mosquitoes via Anthrone Tests
Daniel A.H. Peach
2 Assessment of Mosquito Pollination
Daniel A.H. Peach
CHAPTER 11 INTRODUCTION
Mosquito Blood Meal Analysis
Lawrence E. Reeves and Nathan D. Burkett-Cadena
PROTOCOLS
1 Preservation of Field-Collected Mosquito Blood Meals
Lawrence E. Reeves and Nathan D. Burkett-Cadena
2 Extracting DNA from Preserved Mosquito Blood Meals
Lawrence E. Reeves and Nathan D. Burkett-Cadena
3 Amplification and Identification of Vertebrate Host Cytochrome c Oxidase Subunit I (COI) DNA Barcoding Templates from Mosquito Blood Meals
Lawrence E. Reeves and Nathan D. Burkett-Cadena
SECTION 3. HOST-SEEKING EXPERIMENTS
CHAPTER 12 INTRODUCTION
Quantifying Mosquito Attraction Behavior Using Olfactometry
John S. Castillo, Anthony J. Bellantuono, and Matthew DeGennaro
PROTOCOLS
1 Building a Uniport Olfactometer to Assess Mosquito Responses to Odors
John S. Castillo, Anthony J. Bellantuono, and Matthew DeGennaro
2 Quantifying Mosquito Attraction Using a Uniport Olfactometer
John S. Castillo, Anthony J. Bellantuono, and Matthew DeGennaro
CHAPTER 13 INTRODUCTION
Quantifying Aedes aegypti Host Odor Preference Using a Two-Port Olfactometer
Hillery C. Metz, Jessica L. Zung, and Carolyn S. McBride
PROTOCOL
1 An Assay for Quantifying Aedes aegypti Host Odor Preference Using a Two-Port Olfactometer
Hillery C. Metz, Jessica L. Zung, and Carolyn S. McBride
CHAPTER 14 INTRODUCTION
Quantifying Mosquito Host Preference
Diego Giraldo and Conor J. McMeniman
PROTOCOLS
1 A Behavioral Assay to Quantify Odor-Guided Thermotaxis with Anopheles gambiae under Semi-Field Conditions
Diego Giraldo and Conor J. McMeniman
2 Quantification of Anopheles gambiae Olfactory Preferences under Semi-Field Conditions
Diego Giraldo and Conor J. McMeniman
CHAPTER 15 INTRODUCTION
Key Features and Considerations for Using Automated Long-Term Monitoring of Heat-Seeking Behavior of Mosquitoes
Chisako Sakuma, Emi Maekawa, and Hirotaka Kanuka
PROTOCOL
1 Automated Long-Term Monitoring of the Heat-Seeking Behavior of Mosquitoes
Chisako Sakuma, Emi Maekawa, and Hirotaka Kanuka
SECTION 4. MATING ASSESSMENT
CHAPTER 16 INTRODUCTION
Acoustic-Related Mating Behavior in Tethered and Free-Flying Mosquitoes
Garrett P. League, Catalina Alfonso-Parra, Hoover Pantoja-Sánchez, and Laura C. Harrington
PROTOCOL
1 Recording and Analysis of Mosquito Acoustic-Related Mating Behavior
Hoover Pantoja-Sánchez, Garrett P. League, Laura C. Harrington, and Catalina Alfonso-Parra
CHAPTER 17 INTRODUCTION
Insemination in Aedes aegypti and Aedes albopictus
Dhwani Parsana, Ferdinand Nanfack-Minkeu, and Laura K. Sirot
PROTOCOL
1 Determining the Insemination Status of Aedes Mosquitoes
Dhwani Parsana, Ferdinand Nanfack-Minkeu, and Laura K. Sirot
SECTION 5. EGG AND OVIPOSITION STUDIES
CHAPTER 18 INTRODUCTION
Mosquito Egg Development and Eggshell Formation
Jun Isoe, Michael A. Riehle, and Roger L. Miesfeld
PROTOCOLS
1 Visualization of Apoptotic Ovarian Follicles during Aedes aegypti Mosquito Egg Maturation by Fluorescent Imaging Studies
Jun Isoe, Roger L. Miesfeld, and Michael A. Riehle
2 Identification of Mosquito Eggshell Proteins from Aedes aegypti by Liquid Chromatography with Tandem Mass Spectrometry (LC–MS/MS) Proteomic Analysis
Jun Isoe, Krishna Parsawar, Cynthia L. David, Michael A. Riehle, and Roger L. Miesfeld
CHAPTER 19 INTRODUCTION
Measuring Oviposition Preference in Aedes aegypti Mosquitoes
Yunusa Garba Mohammed, Nicholas Kristoff Tochor, and Benjamin J. Matthews
PROTOCOLS
1 Evaluating Egg-Laying Preference of Individual Aedes aegypti Mosquitoes
Nicholas Kristoff Tochor, Yunusa Garba Mohammed, and Benjamin J. Matthews
2 Automated Egg-Counting Approaches for Aedes aegypti Oviposition Experiments
Nicholas Kristoff Tochor, Yunusa Garba Mohammed, and Benjamin J. Matthews
SECTION 6. FLIGHT AND NAVIGATION ANALYSIS
CHAPTER 20 INTRODUCTION
Retrospective Review of and Introduction to the Analysis of Mosquito Optomotor Responses
Clément Vinauger and Jeffrey A. Riffell
PROTOCOL
1 Tethered Preparation for the Analysis of Mosquito Visual-Motor Responses Using Modular Visual Displays
Clément Vinauger and Jeffrey A. Riffell
CHAPTER 21 INTRODUCTION
Experiments and Analysis of Mosquito Flight Behaviors in a Wind Tunnel: An Introduction
Diego Alonso San Alberto, Claire Rusch, and Jeffrey A. Riffell
PROTOCOL
1 Conducting an Analysis of Mosquito Flight Behaviors in a Wind Tunnel
Diego Alonso San Alberto, Claire Rusch, and Jeffrey A. Riffell
CHAPTER 22 OVERVIEW
Wind Tunnels and Airflow-Driven Assays: Methods for Establishing the Cues and Orientation Mechanisms That Modulate Female Mosquito Attraction to Human Hosts
Ring T. Cardé
CHAPTER 23 INTRODUCTION
Using Videography to Study the Biomechanics and Behavior of Freely Moving Mosquitoes
Andrew K. Dickerson, Florian T. Muijres, and Remco Pieters
PROTOCOLS
1 Designing a Generic Videography Experiment for Studying Mosquito Behavior
Florian T. Muijres, Andrew K. Dickerson, and Remco Pieters
2 Real-Time Tracking of Multiple Moving Mosquitoes
Andrew D. Straw, Remco Pieters, and Florian T. Muijres
3 Tracking the Body, Wing, and Leg Kinematics of Moving Mosquitoes
Tyson L. Hedrick, Andrew K. Dickerson, Florian T. Muijres, and Remco Pieters
4 Quantifying and Analyzing Mosquito Movement from Video Tracking Results
Florian T. Muijres
SECTION 7. PHYSIOLOGY
CHAPTER 24 INTRODUCTION
Mosquito (Diptera: Culicidae) Vision and Associated Electrophysiological Techniques
Daniel A.H. Peach and Adam J. Blake
PROTOCOL
1 Measuring Mosquito Spectral Sensitivity Using Electroretinograms
Daniel A.H. Peach and Adam J. Blake
CHAPTER 25 INTRODUCTION
Mosquito Electroantennography
Chloé Lahondère
PROTOCOL
1 Mosquito Electroantennogram Recordings
Chloé Lahondère
CHAPTER 26 INTRODUCTION
Taste Sensory Responses in Mosquitoes
Adriana Medina Lomelí and Anupama Arun Dahanukar
PROTOCOL
1 Single-Sensillum Taste Recordings in Mosquitoes
Adriana Medina Lomelí and Anupama Arun Dahanukar
CHAPTER 27 INTRODUCTION

Identification of Bioactive Volatile Organic Compounds Using Combined Gas Chromatography and Single-Sensillum Recording
Rickard Ignell, Majid Ghaninia, and Sharon Rose Hill
PROTOCOL
1 Combined Gas Chromatography and Single-Sensillum Recording for Mosquitoes
Rickard Ignell, Majid Ghaninia, and Sharon Rose Hill
CHAPTER 28 INTRODUCTION
GCaMP Imaging in Mosquitoes: Central Nervous System
Zhilei Zhao, Lukas Weiss, and Carolyn S. McBride
PROTOCOL
1 Two-Photon Calcium Imaging in the Brain of Aedes aegypti Mosquitoes
Zhilei Zhao, Lukas Weiss, and Carolyn S. McBride
CHAPTER 29 INTRODUCTION
Genetically Encoded Calcium Indicators for Functional Imaging of Mosquito Olfactory Neurons
Ali Afify and Christopher J. Potter
PROTOCOL
1 Calcium Imaging of Anopheles coluzzii Mosquito Antennae Expressing the Calcium Indicator GCaMP6f
Ali Afify and Christopher J. Potter
CHAPTER 30 INTRODUCTION
Acoustic Physiology in Mosquitoes
Matthew P. Su, Marta Andrés, Marcos Georgiades, Judit Bagi, and Joerg T. Albert
PROTOCOLS
1 Immunohistochemical Staining of the Mosquito Ear
Marta Andrés, Judit Bagi, and Joerg T. Albert
2 Electrophysiological Measurements of Compound Action Potential Responses from the Antennal Nerve in Response to Stimulation
Matthew P. Su and Joerg T. Albert
3 Recording and Extraction of Mosquito Flight Tones
Marcos Georgiades and Joerg T. Albert
4 Mosquito Phonotaxis Assay
Marcos Georgiades and Joerg T. Albert
CHAPTER 31 INTRODUCTION
Physiological and Behavioral Characterization of Larval Olfaction in the Malaria Vector Mosquito Anopheles coluzzii
Feng Liu, Huahua Sun, and Laurence J. Zwiebel
PROTOCOLS
1 Examining Peripheral Electrophysiological Responses of the Larval Antennal Sensory Cone of Anopheles coluzzii to Volatile Odorants
Feng Liu, Huahua Sun, and Laurence J. Zwiebel
2 Cup and Pan Behavioral Assays for Assessing Anopheles coluzzii Larval Volatile Responses
Feng Liu, Huahua Sun, and Laurence J. Zwiebel
SECTION 8. GENETIC TECHNIQUES
CHAPTER 32 INTRODUCTION
Mosquito Embryo Microinjection
Robert A. Harrell II
PROTOCOL
1 Mosquito Embryo Microinjection under Halocarbon Oil or in Aqueous Solution
Robert A. Harrell II
CHAPTER 33 INTRODUCTION
Mosquito Transposon-Mediated Transgenesis
Vanessa Bottino-Rojas and Anthony A. James
PROTOCOL
1 Generating and Validating Transgenic Mosquitoes with Transposon-Mediated Transgenesis
Vanessa Bottino-Rojas and Anthony A. James
CHAPTER 34 INTRODUCTION
Design and Validation of Guide RNAs for CRISPR–Cas9 Genome Editing in Mosquitoes
Ivan Hok Yin Lo and Benjamin J. Matthews
PROTOCOL
1 Validating Single-Guide RNA for Aedes aegypti Gene Editing
Ivan Hok Yin Lo and Benjamin J. Matthews
CHAPTER 35 INTRODUCTION
Oral RNAi for Gene Silencing in Mosquitoes: From the Bench to the Field
Keshava Mysore, Limb K. Hapairai, Jacob S. Realey, Longhua Sun, Joseph B. Roethele, and Molly Duman-Scheel
PROTOCOL
1 Sugar-Baited Delivery of Small Interfering RNA for Gene Silencing in Adult Mosquitoes
Keshava Mysore, Limb K. Hapairai, Jacob S. Realey, Longhua Sun, Joseph B. Roethele, and Molly Duman-Scheel
CHAPTER 36 OVERVIEW
Genetic Toolbox Approaches in Mosquitoes
Olena Riabinina, Matthew Quinn, and Joe P. Whitehead
CHAPTER 37 INTRODUCTION
CRISPR–Cas9 Methods and Key Considerations in the Production of Aedes aegypti Mutant Strains
Ruichen Sun, Robyn Raban, and Omar S. Akbari
PROTOCOL
1 Generating Aedes aegypti Mutant Strains with Transgenic Cas9
Ruichen Sun, Robyn Raban, and Omar S. Akbari
CHAPTER 38 INTRODUCTION
Techniques for Identifying and Sorting Transgenic Mosquito Larvae
Eric Marois
PROTOCOLS
1 Screening Mosquito Larvae under a Fluorescence Binocular Microscope
Eric Marois
2 Sorting Mosquito Larvae with a COPAS Machine
Eric Marois
3 Selecting Transgenic Mosquito Larvae with Puromycin
Eric Marois
SECTION 9. IMAGING AND ANALYSIS OF GENE EXPRESSION
CHAPTER 39 INTRODUCTION
The Golgi Method
Rickard Ignell and Sharon Rose Hill
PROTOCOL
1 Golgi Silver Staining of Mosquito Central Neuropils
Rickard Ignell and Sharon Rose Hill
CHAPTER 40 INTRODUCTION
Introduction to Techniques Used to Study Mosquito Neuroanatomy and Neural Circuitry
Florence V. Guerina, Ameya P. Patkar, and Meg A. Younger
PROTOCOLS
1 Whole-Mount Immunofluorescent Labeling of the Mosquito Central Nervous System
Meg A. Younger
2 Dextran Amine–Conjugated Neural Tracing in Mosquitoes
Meg A. Younger
CHAPTER 41 INTRODUCTION
Mapping Transcript Cell-Specific Localization and Protein Subcellular Localization in the Adult Mosquito Aedes aegypti
Farwa Sajadi, Salwa Afifi, Britney Picinic, and Jean-Paul V. Paluzzi
PROTOCOLS
1 Examining Cell-Specific Localization in Aedes aegypti Tissues with Fluorescence In Situ Hybridization
Farwa Sajadi and Jean-Paul V. Paluzzi
2 Examining Protein Localization in Aedes aegypti Cells, Tissues, and Organs: Whole-Mount Immunohistochemistry
Salwa Afifi, Farwa Sajadi, and Jean-Paul V. Paluzzi
3 Examining Apical or Basolateral Protein Localization in Aedes aegypti Tissues: Cross-Section Immunohistochemistry
Farwa Sajadi, Britney Picinic, Salwa Afifi, and Jean-Paul V. Paluzzi
4 Membrane Protein Fractionation and Analysis through Western Blot in Aedes aegypti Malpighian Tubules
Britney Picinic and Jean-Paul V. Paluzzi
CHAPTER 42 INTRODUCTION
Performing Immunohistochemistry in Mosquito Salivary Glands
Ines Martin-Martin, Thiago Luiz Alves E. Silva, Adeline E. Williams, Joel Vega-Rodriguez, and Eric Calvo
PROTOCOL
1 A Simple Method for Immunohistochemistry and Imaging of Mosquito Salivary Glands
Ines Martin-Martin, Thiago Luiz Alves E. Silva, Adeline E. Williams, Joel Vega-Rodriguez, and Eric Calvo
CHAPTER 43 INTRODUCTION
RNA In Situ Hybridization and Immunohistochemistry to Visualize Gene Expression in Peripheral Chemosensory Tissues of Mosquitoes
Margaret Herre and Chloe Greppi
PROTOCOLS
1 Cryosectioning and Immunohistochemistry of Peripheral Tissues of Mosquitoes
Chloe Greppi
2 Immunostaining Whole-Mount Olfactory Tissues of Mosquitoes
Margaret Herre
3 RNA In Situ Hybridization of Whole-Mount Mosquito Olfactory Tissues
Margaret Herre
CHAPTER 44 INTRODUCTION
Osmoregulatory Challenges Faced by Mosquitoes and Techniques for Studying the Transporters Involved in Their Adaptations
Andrea C. Durant and Andrew Donini
PROTOCOL
1 Localizing Protein Expression of Transporters in Epithelial Cell Membranes of Mosquitoes
Andrea C. Durant and Andrew Donini
CHAPTER 45 INTRODUCTION
Visualization of the Linear and Spatial Organization of Chromosomes in Mosquitoes
Jiangtao Liang, Simon M. Bondarenko, Igor V. Sharakhov, and Maria V. Sharakhova
PROTOCOLS
1 Obtaining Polytene, Meiotic, and Mitotic Chromosomes from Mosquitoes for Cytogenetic Analysis
Jiangtao Liang, Simon M. Bondarenko, Igor V. Sharakhov, and Maria V. Sharakhova
2 Visualization of Polytene Chromatin in Mosquito Cell Nuclei Using Three-Dimensional Fluorescence In Situ Hybridization
Simon M. Bondarenko, Jiangtao Liang, Maria V. Sharakhova, and Igor V. Sharakhov
SECTION 10. INSECTICIDE RESISTANCE
CHAPTER 46 INTRODUCTION
Assays to Measure Insecticide Toxicity and Insecticide Resistance in Mosquitoes
Nannan Liu, Yifan Wang, and Ting Li
PROTOCOLS
1 Mosquito Larval Bioassays
Yifan Wang, Ting Li, and Nannan Liu
2 Mosquito Adult Bioassays
Ting Li, Yifan Wang, and Nannan Liu
3 Synergism Study for Investigating Possible Mechanisms of Insecticide Resistance in Mosquitoes
Ting Li, Yifan Wang, and Nannan Liu
Appendix
General Safety and Hazardous Material Information

Index
   

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Angiogenesis: Biology and Pathology, Second Edition


Book Series:  A Cold Spring Harbor Perspectives in Medicine Collection
Subject Area(s):  Developmental BiologyMolecular BiologyCancer Biology

Edited by Diane R. Bielenberg, Harvard Medical School; Patricia A. D'Amore, Harvard Medical School

Due June 2024 • 450 pages (approx.), illustrated, index
Hardcover •
ISBN  978-1-621824-34-3

Buy the print book on Amazon.

Some of our books are temporarily unavailable to order in print format directly from CSHL Press. In the meantime we invite you to order these titles on Amazon. Please contact us with any questions.



  •     Description    
  •     Contents    

Description

The growth of new blood vessels plays an important role in normal development and cancer progression. This new edition updates our understanding of the processes involved and how they may be targeted therapeutically.

Contents

Preliminary
Endothelial Cell Differentiation and Hemogenic Specification
Jordon W. Aragon and Karen K. Hirschi
Human Endothelial Colony-Forming Cells
Juan M. Melero-Martin
Mechanisms of endothelial cell fate determination
Jan Kitajewski, Naiche Adler, and Stephanie Villa Niemczyk
Signal Transduction and Gene Regulation in the Endothelium
Michel V. Levesque and Timothy Hla
Blood vessel patterning
Victoria L. Bautch & Yosuke Mukouyama
Organ vascularization
Ondine Cleaver
Organ-specific endothelium
Ralf Adams
Extracellular Matrix Regulation of Vascular Morphogenesis, Maturation, and Stabilization
George E. Davis and Scott S. Kemp
Endothelial Connexins in Developmental and Pathological Angiogenesis
Jacques-Antoine Haefliger, Paolo Meda, and Florian Alonso
Notch Signaling in Vascular Endothelial and Mural Cell Communications
Michael O'Hare and Joseph F. Arboleda-Velasquez
Endothelial lipid metabolism
Zoltan Arany
Endothelial metabolism
Michael Potente, Joseph Lim, and Jorge Andrade
Angiogenesis and Microvascular Permeability
Ye Zeng and Bingmei M. Fu
Gut Microbiota and the Microvasculature
Klytaimnistra Kiouptsi and Christoph Reinhardt
Beyond Static Pipes: Mechanisms and In Vitro Models of Vascular Aging
Simone Bersini and Martin W. Hetzer
Mechanisms Regulating T Cell-Endothelial Cell Interactions
Pilar Alcaide
Angiogenesis and resolution in inflammation
Dipak Panigrahy and Molly Gilligan
Endothelialitis, Microischemia and Intussusceptive Angiogenesis in COVID-19
Steven J. Mentzer, Maximilian Ackermann, and Danny Jonigk
Epsin Endocytic Adaptor Proteins in Angiogenic and Lymphangiogenic Signaling
Douglas B. Cowan, Hao Wu, and Hong Chen
Prostanoids regulate angiogenesis and lymphangiogenesis in the pathological conditions
Masataka Majima, Kanako Hosono, Yoshiya Ito, and Hideki Amano
Platelets and (Lymph)angiogenesis
Harvey G. Roweth and Elisabeth M. Battinelli
Buttons and Zippers: Endothelial Junctions in Lymphatic Vessels
Peter Baluk and Donald M. McDonald
Lymphatics in Cardiovascular Physiology
Dakshnapriya Balasubbramanian and Brett M. Mitchell
Signaling in lymphangiogenesis
Young-Kwon Hong
Notch Signaling in the Vasculature: Angiogenesis and Angiocrine Functions
Sana S. Hasan and Andreas Fischer
Mechanics of Lymphatic Pumping and Lymphatic Function
Mohammad S. Razavi, Lance L. Munn, and Timothy P. Padera
Lymphedema and Obesity
Christopher L. Sudduth and Arin K. Greene
Lymphatic Tissue and Organ Engineering for in Vitro Modeling and in Vivo Regeneration
Anna M. Kolarzyk, Gigi Wong, and Esak Lee
Lymphangiogenesis and lymphedema
Diane Bielenberg, Yao Gao, and Dakshnapriya Ballasubbramanian
Modeling brain vasculature immune interactions in vitro
Britta Engelhardt, Ruth Lyck, and Hideaki Nishihara
Molecular Mechanisms Driving Lymphedema and Other Lymphatic Anomalies
Xin Geng and R. Sathish Srinivasan
Leukocyte Trafficking in Lymphatic Vessels
Aline Bauer, Hazal Tatliadim, and Cornelia Halin
Lymphatic Clearance and Pump Function
Jerome W. Breslin
Endothelial Cell–Pericyte Interactions in Cerebral Cavernous Malformations (CCM) Pathogenesis
Wang Min and Jenny Huanjiao Zhou
Regulation of the Blood-Brain Barrier in Health and Disease
Cara C. Rada, Kanako Yuki, Jie Ding, and Calvin J. Kuo
Wnt/Frizzled pathway in endothelium
Cécile Couffinhal Duplaa
Lymphatic Vascular Permeability
Joshua Scallan
MicroRNAs in Mechanical Homeostasis
Jeremy A. Herrera and Martin A. Schwartz
Index
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