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Genome Science: A Practical and Conceptual Introduction to Molecular Genetic Analysis in Eukaryotes


Subject Area(s):  BiotechnologyMolecular BiologyGeneticsLaboratory Techniques

By David Micklos, Cold Spring Harbor Laboratory; Bruce Nash, Cold Spring Harbor Laboratory; Uwe Hilgert, University of Arizona

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Download Lab 4.1
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© 2014 • 704 pp., illus (3 4C, 606 B&W), index
Paperback •
ISBN  978-1-621821-09-0

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Description

Genome Science is a textbook and laboratory manual for advanced secondary and post-secondary education. It combines approachable narrative with extensively tested lab exercises that illustrate key concepts of genome biology in humans, invertebrates, and plants. Nineteen labs, organized into four chapters, engage students with both bioinformatics exercises and in vitro experiments. Each chapter also includes an extensive introduction that provides an historical and conceptual framework. This modular structure offers many options for enhancing existing courses, starting new courses, or supporting student research projects. The book is complete with advice for instructors, laboratory planning guidelines, recipes for solutions, and answers to student questions.

Contents

1. Genome as Information
Introduction
Lab 1.1 Annotating Genomic DNA
Lab 1.2 Detecting a Lost Chromosome
Lab 1.3 Comparing Diversity in Eukaryotes
Lab 1.4 Determining the Transposon Content in Grasses
Lab 1.5 Identifying GAI Gene Family Members in Plants
Lab 1.6 Discovering Evidence for Pseudogene Function
Laboratory Planning and Preparation
Answers to Questions
 
2. The Human Genome
Introduction
Lab 2.1 Using Mitochondrial DNA Polymorphisms in Evolutionary Biology
Lab 2.2 Using an Alu Insertion Polymorphism to Study Human Populations
Lab 2.3 Using a Single-Nucleotide Polymorphism to Predict Bitter-Taste Ability
Laboratory Planning and Preparation
Recipes for Reagents and Stock Solutions
Answers to Questions
 
3. Plant Genomes
Introduction
Lab 3.1 Detecting a Transposon in Corn
Lab 3.2 Detecting a Transposon in Arabidopsis
Lab 3.3 Linkage Mapping a Mutation
Lab 3.4 Detecting Genetically Modified Foods by Polymerase Chain Reaction
Lab 3.5 Using DNA Barcodes to Identify and Classify Living Things
Lab 3.6 Detecting Epigenetic DNA Methylation in Arabidopsis
Laboratory Planning and Preparation
Recipes for Reagents and Stock Solutions
Answers to Questions
 
 
4. Genome Function
Introduction
Lab 4.1 Culturing and Observing C. elegans
Lab 4.2 Using E. coli Feeding Strains to Induce RNAi and Knock Down Genes
Lab 4.3 Examining the RNAi Mechanism
Lab 4.4 Constructing an RNAi Feeding Vector
Laboratory Planning and Preparation
Recipes for Reagents and Stock Solutions
Answers to Questions
 
Cautions Appendix
Equipment Appendix
Subject Index