Gene Transfer: Delivery and Expression of DNA and RNA, A Laboratory Manual

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Click to Enlarge				Gene Transfer: Delivery and Expression of DNA and RNA, A Laboratory Manual Subject Area(s): Developmental Biology; Gene Therapy; Cell Biology; Molecular Biology; Laboratory Techniques Edited by Theodore Friedmann, University of California, San Diego; John Rossi, Beckman Research Institute of the City of Hope, Duarte, California View sample pages from the book here — To view sample protocol click here.

				© 2007 • 793 pp., illus., appendix, index Paperback • ~~$167~~ 33.40 ISBN 978-087969765-5 You save: 80% This title also available in: Hardcover

Description Contents Reviews Related Titles Description Understanding gene function and regulation requires rigorous testing in live cells and organisms. Recent advances have provided a variety of new strategies for delivering DNA and RNA into cells and probing their expression, as well as new clinical applications that rely upon the introduction of genetic material. The vast number of available techniques for clinical and laboratory research often makes selecting the optimal method a difficult process. Gene Transfer: Delivery and Expression of DNA and RNA provides the first comprehensive guide to technical approaches for delivering nucleic acids into cells and organisms and of ensuring (even manipulating) appropriate expression. The detailed, step-by-step protocols cover a variety of methods, both well established and newly evolving. These include viral and nonviral methods of gene delivery, transgenic approaches, strategies for the regulation of transgene expression, and modification of the host response. The introductory matter to each chapter includes concise technical and theoretical discussions with considerations for selection of the appropriate system and strategies for delivery. Contents 1. Introduction T. Friedmann and J. Rossi VIRAL VECTORS 2. Retroviral Vectors K. Cornetta, K.E. Pollok, and A.D. Miller 3. Development of Lentiviral Vectors Expressing siRNA G. Tiscornia, O. Singer, and I.M. Verma 4. HIV–2 Vectors in Human Gene Therapy: Design, Construction, and Therapeutic Potential K.V. Morris and F. Wong–Staal 5. SIV Vectors as Vehicles for DNA Delivery E. Verhoeyen, F.–L. Cosset, and D. Nègre 6. Production and Use of Feline Immunodeficiency Virus–based Lentiviral Vectors D.T. Saenz, R. Barraza, N. Loewen, W. Teo, and E.M. Poeschla 7. Lentivirus Transduction of Hematopoietic Cells M.–J. Li and J.J. Rossi 8. Spleen Necrosis Virus–based Vectors Z. Parveen, M. Mukhtar, and R.J. Pomerantz 9. Foamy Virus Vector Production and Transduction of Hematopoietic Cells N.C. Josephson and D.W. Russell 10. Simian Foamy Virus Type–1 Vectors J. Park and A. Mergia 11. Generation of VSV–G–pseudotyped Retroviral Vectors J.–K. Yee 12. Targeted Gene Transfer with Surface–engineered Lentiviral Vectors E. Verhoeyen and F.–L. Cosset 13. Preparation of Pseudotyped Lentiviral Vectors Resistant to Inactivation by Serum Complement G.H. Guibinga and T. Friedmann 14. Generation of 2A Peptide–linked Multicistronic Vectors A.L. Szymczak–Workman, K.M. Vignali, and D.A.A. Vignali 15. Construction of First–generation Adenoviral Vectors P. J. Ross and R.J. Parks 16. Production and Characterization of Helper–dependent Adenoviral Vectors D.J. Palmer and P. Ng 17. Cell and Tissue Targeting Y. Kawakami and D.T. Curiel 18. Stable Producer Cell Lines for AAV Assembly G. Chadeuf and A. Salvetti 19. Strategies for the Design of Hybrid Adeno–associated Virus Vectors A. Asokan and R.J. Samulski 20. Recombinant Herpes Simplex Virus Vectors W.F. Goins, D. M. Krisky, J.B. Wechuck, D. Wolfe, S. Huang, and J.C. Glorioso 21. Herpes Simplex Virus Type–1–derived Amplicon Vectors W.J. Bowers and H.J. Federoff 22. γ–2 Herpesvirus Saimiri–based Vectors A. Whitehouse 23. Gene Delivery Using HSV/AAV Hybrid Amplicon Vectors O. Saydam, D.L. Glauser, and C. Fraefel 24. Polyomaviruses: SV40 D.S. Strayer, C. Mitchell, D.A. Maier, and C.N. Nichols 25. SV40 In Vitro Packaging: A Pseudovirion Gene Delivery System C. Kimchi–Sarfaty and M.M. Gottesman 26. Baculovirus–based Display and Gene Delivery Systems A.R. Mäkelä, W. Ernst, R. Grabherr, and C. Oker–Blom 27. Safe, Simple, and High–capacity Gene Delivery into Insect and Vertebrate Cells by Recombinant Baculoviruses K.J. Airenne, O.H. Laitinen, A. J. Mähönen, and S. Ylä–Herttuala 28. Alphaviruses: Semliki Forest Virus and Sindbis Virus as Gene Delivery Vectors K. Lundstrom 29. Gene Transfer into Mammalian Cells Using Targeted Filamentous Bacteriophage A. Baird 30. Selection, Isolation, and Identification of Targeting Peptides for Ligand–directed Gene Delivery M. Trepel, W. Arap, and R. Pasqualini 31. Rescue and Propagation of Tropism–modified Measles Viruses T. Nakamura and S.J. Russell 32. Picornavirus–based Expression Vectors S. Mueller and E. Wimmer 33. Reverse Genetics of Influenza Viruses G.A. Marsh and P. Palese NONVIRAL TECHNIQUES AND VECTORS 34. An Overview of Condensing and Noncondensing Polymeric Systems for Gene Delivery D.B. Shenoy and M.M. Amiji 35. Transfection of Hippocampal Neurons with Plasmid DNA Using Calcium Phosphate Coprecipitation B. Goetze and M. Kiebler 36. Gene Delivery to Skin Using Biolistics W.C. Heiser 37. Optimizing Electrotransfection of Mammalian Cells In Vitro S. Li 38. Micro In Utero Electroporation for Efficient Gene Targeting in Mouse Embryos T. Shimogori 39. Lipoplex and LPD Nanoparticles for In Vivo Gene Delivery S.–D. Li, S. Li, and L. Huang 40. Bioresponsive Targeted Charge Neutral Lipid Vesicles for Systemic Gene Delivery W. Li and F.C. Szoka, Jr. 41. HVJ Liposomes and HVJ Envelope Vectors Y. Kaneda 42. Polylysine Copolymers for Gene Delivery S.W. Kim 43. PEI Nanoparticles for Targeted Gene Delivery F. Alexis, J. Zeng, and S. Wang 44. Cyclodextrin–containing Polycations for Nucleic Acid Delivery J.D. Heidel 45. Bionanocapsules Using the Hepatitis B Virus Envelope L Protein T. Yamada, J. Jung, M. Seno, A. Kondo, M. Ueda, K. Tanizawa, and S. Kuroda 46. Formulations of Solid Lipid Nanoparticles for Transfection of Mammalian Cells In Vitro C. Rudolph and J. Rosenecker 47. PEGylated Poly–l–lysine DNA Nanoparticles P.B. Davis and T.H. Kowalczyk 48. Water–soluble Lipopolymers and Lipopeptides for Nucleic Acid Delivery R.I. Mahato, Z. Ye, and S.W. Kim 49. Cationic Polysaccharides for DNA Delivery I. Yudovin–Farber, H. Eliyahu, and A.J. Domb 50. Sustained Release of Plasmid DNAs Encoding Platelet–derived Growth Factor and Hyaluronan Synthase 2 from Cross–linked Hyaluronan Matrices and Films W. Chen 51. Linear Polyethylenimine: Synthesis and Transfection Procedures for In Vitro and In Vivo M. Ogris and E. Wagner 52. Protein Nanospheres for Gene Delivery: Preparation and In Vitro Transfection Studies with Gelatin Nanoparticles S. Kommareddy and M.M. Amiji 53. Vesicular Stomatitis Virus–G Conjugate A. Miyanohara 54. High–throughput Methods for Screening Polymeric Transfection Reagents G.T. Zugates, D.G. Anderson, and R. Langer 55. Poly(Lactic Acid) and Poly(Ethylene Oxide) Nanoparticles as Carriers for Gene Delivery N. S. Csaba, A. Sánchez, and M. J. Alonso 56. Biodegradable Nanoparticles J.K. Vasir and V. Labhasetwar 57. Transposon–mediated Delivery of Small Interfering RNA: Sleeping Beauty Transposon B.S. Fletcher 58. Efficient DNA Delivery into Mammalian Cells by Displaying the TAT Transduction Domain on Bacteriophage λ J. Wadia, A. Eguchi, and S.F. Dowdy 59. Cell–penetrating Peptide–mediated Delivery of Peptide Nucleic Acid Oligomers P. Lundberg, K. Kilk, and Ü. Langel REGULATION OF TRANSGENE EXPRESSION 60. Conditional Mutagenesis of the Genome Using Site–specific DNA Recombination K. Ohtsubo and J.D. Marth 61. Expression and Validation of Ribozyme and Short Hairpin RNA in Mammalian Cells M. Amarzguioui 62. Mifepristone–inducible Gene Regulatory System K. Schillinger, X. Ye, S. Tsai, and B.W. O'Malley 63. Dimerizer–mediated Regulation of Gene Expression V.M. Rivera, L. Berk, and T. Clackson 64. RheoSwitch System: A Highly Sensitive Ecdysone Receptor–based Gene Regulation System Induced by Synthetic Small–molecule Ligands P. Kumar and A. Katakam 65. Site–specific Integration with Phage φC31 Integrase R.T. Hillman and M.P. Calos 66. Creating Zinc Finger Nucleases to Manipulate the Genome in a Site–specific Manner Using a Modular–assembly Approach M. Porteus SPECIALIZED TECHNIQUES OF GENE AND VECTOR DELIVERY 67. Assembly of De Novo Bacterial Artificial Chromosome–based Human Artificial Chromosomes J. Basu and H.F. Willard 68. Delivery of Naked DNA Using Hydrodynamic Injection Techniques D.L. Lewis, M. Noble, J. Hegge, and J. Wolff 69. Nonviral Gene Transfer across the Blood–brain Barrier with Trojan Horse Liposomes W. M. Pardridge 70. Sonoporation: An Efficient Technique for the Introduction of Genes into Chick Embryos S. Ohta, O. Yukiko, K. Suzuki, M. Kamimura, K. Tachibana, and G. Yamada 71. Genetic Manipulation of Mammalian Cells by Microinjection D.W. Rose 72. Magnetofection C. Plank and J. Rosenecker 73. Photochemical Internalization for Light–directed Gene Delivery A. Bonsted, A. Høgset, E. Wagner, and K. Berg TRANSGENIC APPROACHES 74. Pronuclear Microinjection in Mice W. Tsark 75. Knockdown Transgenic Mice Generated by Silencing Lentiviral Vectors O. Singer, G. Tiscornia, and I.M. Verma APPENDIX Cautions Subject Index Reviews “This volume is a very comprehensive and practical guide describing all the virally, chemically and physically mediated gene transfer techniques I have come across and a few more!...It is a very useful resource.” —Society for General Microbiology Quarterly Related Titles Gene Transfer: Delivery and Expression of DNA and RNA, A Laboratory Manual [Hardcover] The Development of Human Gene Therapy [Cloth]