Budding Yeast: A Laboratory Manual

Subject Area(s): Yeast; Molecular Biology; Biochemistry; Cytology

Edited by Brenda Andrews, University of Toronto; Charles Boone, University of Toronto; Trisha N. Davis, University of Washington; Stanley Fields, Howard Hughes Medical Institute and University of Washington

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Preface
Index
Historical Evolution of Laboratory Strains of Saccharomyces cerevisiae
Sample Protocol: Analysis of Replicating Yeast Chromosomes by DNA Combing

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Description
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Description

Over the past century, studies of the budding yeast Saccharomyces cerevisiae have helped to unravel principles of nearly every aspect of eukaryotic cell biology—from metabolism and molecular genetics to cell division and differentiation. Thanks to its short generation time, ease of genetic manipulation, and suitability for high-throughput studies, yeast remains the focus of research in a vast number of laboratories worldwide.

This laboratory manual provides a comprehensive collection of experimental procedures that continue to make budding yeast an informative model. The contributors describe methods for culturing and genetically modifying yeast, strategies and tools (e.g., gene deletion collections) for functional analyses, approaches for characterizing cell structure and morphology, and techniques to probe the modifications and interactions of various cellular constituents (e.g., using one- and two-hybrid screens). Strategies for studying metabolomics, complex traits, and evolution in yeast are also covered, as are methods to isolate and investigate new strains of yeast from the wild.

Several additional chapters are devoted to bioinformatics tools and resources for yeast biology (e.g., the Saccharomyces Genome Database). This manual is therefore an essential resource for all researchers, from graduate level upward, who use budding yeast to explore the intricate workings of cells.

Preface
CHAPTER 1 Historical Evolution of Laboratory Strains of Saccharomyces cerevisiae
INTRODUCTION
Historical Evolution of Laboratory Strains of Saccharomyces cerevisiae: Edward J. Louis
CHAPTER 2 Classical Genetics with Saccharomyces cerevisiae
INTRODUCTION
Classical Genetics with Saccharomyces cerevisiae: David C. Amberg and Daniel J. Burke
CHAPTER 3 Analysis of Recombination and Chromosome Structure during Yeast Meiosis
INTRODUCTION
Analysis of Recombination and Chromosome Structure during Yeast Meiosis: G. Valentin Börner and Rita S. Cha
PROTOCOLS: 1 Analysis of Yeast Sporulation Efficiency, Spore Viability, and Meiotic Recombination on Solid Medium; G. Valentin Börner and Rita S. Cha; 2 Induction and Analysis of Synchronous Meiotic Yeast Cultures; G. Valentin Börner and Rita S. Cha; 3 Analysis of Meiotic Recombination and Homolog Interaction during Yeast Meiosis; G. Valentin Börner and Rita S. Cha
CHAPTER 4 Investigating Filamentous Growth and Biofilm/Mat Formation in Budding Yeast
INTRODUCTION
Investigating Filamentous Growth and Biofilm/Mat Formation in Budding Yeast: Paul J. Cullen
PROTOCOLS
1 The Plate-Washing Assay: A Simple Test for Filamentous Growth in Budding Yeast: Paul J. Cullen
2 Biofilm/Mat Assays for Budding Yeast: Paul J. Cullen
3 Evaluating Filamentous Yeast Growth at the Single-Cell Level: Paul J. Cullen
4 Evaluating the Activity of the Filamentous Growth Mitogen-Activated Protein Kinase Pathway in Yeast: Paul J. Cullen
CHAPTER 5 Construction of Multi-fragment Plasmids by Homologous Recombination in Yeast
INTRODUCTION
Construction of Multi-fragment Plasmids by Homologous Recombination in Yeast: Jolanda S. van Leeuwen, Brenda J. Andrews, Charles Boone, and Guihong Tan
PROTOCOLS
1 Rapid and Efficient Plasmid Construction by Homologous Recombination in Yeast: Jolanda van Leeuwen, Brenda Andrews, Charles Boone, and Guihong Tan
CHAPTER 6 Single-Molecule Analysis of Replicating Yeast Chromosomes
INTRODUCTION
Single-Molecule Analysis of Replicating Yeast Chromosomes: David Gallo, Gang Wang, Christopher M. Yip, and Grant W. Brown
PROTOCOL
1 Analysis of Replicating Yeast Chromosomes by DNA Combing: David Gallo, Gang Wang, Christopher M. Yip, and Grant W. Brown
CHAPTER 7 Measuring Chromatin Structure in Budding Yeast
INTRODUCTION
Measuring Chromatin Structure in Budding Yeast: Jon-Matthew Belton and Job Dekker
PROTOCOLS
1 Chromosome Conformation Capture (3C) in Budding Yeast: Jon-Matthew Belton and Job Dekker
2 Randomized Ligation Control for Chromosome Conformation Capture: Jon-Matthew Belton and Job Dekker
3 Chromosome Conformation Capture Carbon Copy (5C) in Budding Yeast: Jon-Matthew Belton and Job Dekker
4 Hi-C in Budding Yeast: Jon-Matthew Belton and Job Dekker
CHAPTER 8 MX Cassettes for Knocking Out Genes in Yeast
INTRODUCTION
MX Cassettes for Knocking Out Genes in Yeast: John H. McCusker
PROTOCOLS
1 Introducing MX Cassettes into Saccharomyces cerevisiae: John H. McCusker
2 Popping Out MX Cassettes from Saccharomyces cerevisiae: John H. McCusker
CHAPTER 9 Multi-Purpose Transposon-Insertion Libraries in Yeast
INTRODUCTION
Multi-Purpose Transposon-Insertion Libraries in Yeast: Anuj Kumar
PROTOCOL
1 Using Yeast Transposon-Insertion Libraries for Phenotypic Screening and Protein Localization: Anuj Kumar
CHAPTER 10 Functional Genomics Using the Saccharomyces cerevisiae Yeast Deletion Collections
INTRODUCTION
Functional Genomics Using the Saccharomyces cerevisiae Yeast Deletion Collections: Corey Nislow, Lai Hong Wong, Amy Huei-Yi Lee, and Guri Giaever
PROTOCOL
1 Functional Profiling Using the Yeast Deletion Collections: Corey Nislow, Lai Hong Wong, Amy Huei-Yi Lee, and Guri Giaever
CHAPTER 11 Deep Mutational Scanning: A Highly Parallel Method to Measure the Effects of Mutation on Protein Function
INTRODUCTION
Deep Mutational Scanning: A Highly Parallel Method to Measure the Effects of Mutation on Protein Function: Lea M. Starita and Stanley Fields
PROTOCOLS
1 Deep Mutational Scanning: Library Construction, Functional Selection, and High-Throughput Sequencing: Lea M. Starita and Stanley Fields
2 Deep Mutational Scanning: Calculating Enrichment Scores for Protein Variants from DNA Sequencing Output Files: Lea M. Starita and Stanley Fields
CHAPTER 12 Examination and Disruption of the Yeast Cell Wall
INTRODUCTION
Examination and Disruption of the Yeast Cell Wall: Hiroki Okada, Keiko Kono, Aaron M. Neiman, and Yoshikazu Ohya
PROTOCOLS
1 Fluorescent Labeling of Yeast Cell Wall Components: Hiroki Okada and Yoshikazu Ohya
2 Assay for Spore Wall Integrity Using a Yeast Predator: Hiroki Okada, Aaron M. Neiman, and Yoshikazu Ohya
3 Local and Acute Disruption of the Yeast Cell Surface: Keiko Kono, Hiroki Okada, and Yoshikazu Ohya
CHAPTER 13 Analyzing and Understanding Lipids of Yeast: A Challenging Endeavor
INTRODUCTION
Analyzing and Understanding Lipids of Yeast: A Challenging Endeavor: Sepp D. Kohlwein
PROTOCOLS
1 Lipid Extraction from Yeast Cells: Oskar L. Knittelfelder and Sepp D. Kohlwein
2 Thin Layer Chromatography to Separate Phospholipids and Neutral Lipids from Yeast: Oskar L. Knittelfelder and Sepp D. Kohlwein
3 Derivatization and Gas Chromatography of Fatty Acids from Yeast: Oskar L. Knittelfelder and Sepp D. Kohlwein
4 Quantitative Analysis of Yeast Phospholipids and Sterols by High-Performance Liquid Chromatography-Evaporative Light-Scattering Detection: Oskar L. Knittelfelder and Sepp D. Kohlwein
CHAPTER 14 Methods for Synchronization and Analysis of the Budding Yeast Cell Cycle
INTRODUCTION
Methods for Synchronization and Analysis of the Budding Yeast Cell Cycle: Adam P. Rosebrock
PROTOCOLS
1 Synchronization and Arrest of the Budding Yeast Cell Cycle Using Chemical and Genetic Methods: Adam P. Rosebrock
2 Synchronization of Budding Yeast by Centrifugal Elutriation: Adam P. Rosebrock
3 Analysis of the Budding Yeast Cell Cycle by Flow Cytometry: Adam P. Rosebrock
CHAPTER 15 High Throughput Microscopy-based Screening in Saccharomyces cerevisiae
INTRODUCTION
High Throughput Microscopy-based Screening in Saccharomyces cerevisiae: Erin B. Styles, Helena Friesen, Charles Boone, and Brenda Andrews
PROTOCOLS
1 Liquid Growth of Arrayed Fluorescently Tagged Saccharomyces cerevisiae Strains for Live-Cell High-Throughput Microscopy Screens: Michael J. Cox, Yolanda T. Chong, Charles Boone, and Brenda Andrews
2 Quantification of Cell, Actin, and Nuclear DNA Morphology with High-Throughput Microscope and CalMorph: Hiroki Okada, Shinsuke Ohnuki, and Yoshikazu Ohya
CHAPTER 16 Single-Molecule Total Internal Reflection Fluorescence Microscopy
INTRODUCTION
Single-Molecule Total Internal Reflection Fluorescence Microscopy: Emily M. Kudalkar, Trisha N. Davis, and Charles L. Asbury
PROTOCOLS
1 Coverslip Cleaning and Functionalization for Total Internal Reflection Fluorescence Microscopy: Emily M. Kudalkar, Yi Deng, Trisha N. Davis, and Charles L. Asbury
2 Preparation of Reactions for Imaging with Total Internal Reflection Fluorescence Microscopy: Emily M. Kudalkar, Trisha N. Davis, and Charles L. Asbury
3 Data Analysis for Total Internal Reflection Fluorescence Microscopy: Charles L. Asbury
CHAPTER 17 Building Cell Structures in Three Dimensions: Electron Tomography Methods for Budding Yeast
INTRODUCTION
Building Cell Structures in Three Dimensions: Electron Tomography Methods for Budding Yeast: Eileen T. O’Toole, Thomas H. Giddings, Jr., and Mark Winey
PROTOCOL
1 Cryopreparation and Electron Tomography of Yeast Cells: Eileen T O’Toole, Thomas H. Giddings, Jr., and Mark Winey
CHAPTER 18 The Yeast Two-Hybrid System: A Tool for Mapping Protein-Protein Interactions
INTRODUCTION
The Yeast Two-Hybrid System: A Tool for Mapping Protein-Protein Interactions: Jitender Mehla, J. Harry Caufield, and Peter Uetz
PROTOCOL
1 Mapping Protein-Protein Interactions Using Yeast Two-Hybrid Assays: Jitender Mehla, J. Harry Caufield, and Peter Uetz
CHAPTER 19 Membrane Yeast Two-Hybrid (MYTH) Mapping of Full-length Membrane Protein Interactions
INTRODUCTION
Membrane Yeast Two-Hybrid (MYTH) Mapping of Full-length Membrane Protein Interactions: Jamie Snider and Igor Stagljar
PROTOCOLS
1 Generation and Validation of MYTH Baits: iMYTH and tMYTH Variants: Jamie Snider and Igor Stagljar
2 MYTH Screening: iMYTH and tMYTH Variants: Jamie Snider and Igor Stagljar
CHAPTER 20 Protein-Fragment Complementation Assays for Large-Scale Analysis, Functional Dissection, and Spatiotemporal Dynamic Studies of Protein-Protein Interactions in Living Cells
INTRODUCTION
Protein-Fragment Complementation Assays for Large-Scale Analysis, Functional Dissection, and Spatiotemporal Dynamic Studies of Protein-Protein Interactions in Living Cells: Stephen W. Michnick, Christian R. Landry, Emmanuel D. Levy, Guillaume Diss, Po Hien Ear, Jacqueline Kowarzyk, Mohan K. Malleshaiah, Vincent Messier, and Emmanuelle Tchekanda
PROTOCOLS
1 The Dihydrofolate Reductase Protein-Fragment Complementation Assay: A Survival-Selection Assay for Large-Scale Analysis of Protein-Protein Interactions: Stephen W. Michnick, Emmanuel D. Levy, Christian R. Landry, Jacqueline Kowarzyk, and Vincent Messier
2 Combining the Dihydrofolate Reductase Protein-Fragment Complementation Assay with Gene Deletions to Establish Genotype-to-Phenotype Maps of Protein Complexes and Interaction Networks: Guillaume Diss and Christian R. Landry
3 Dissecting the Contingent Interactions of Protein Complexes with the Optimized Yeast Cytosine Deaminase Protein-Fragment Complementation Assay: Po Hien Ear, Jacqueline Kowarzyk, and Stephen W. Michnick
4 Real-Time Protein-Fragment Complementation Assays for Studying Temporal, Spatial, and Spatiotemporal Dynamics of Protein-Protein Interactions in Living Cells: Mohan Malleshaiah, Emmanuelle Tchekanda, and Stephen W. Michnick
CHAPTER 21 Protein Complex Purification by Affinity Capture
INTRODUCTION
Protein Complex Purification by Affinity Capture: John LaCava, Javier Fernandez-Martinez, Zhanna Hakhverdyan, and Michael P. Rout
PROTOCOLS
1 Optimized Affinity Capture of Yeast Protein Complexes: John LaCava, Javier Fernandez-Martinez, Zhanna Hakhverdyan, and Michael P. Rout
2 Native Elution of Yeast Protein Complexes Obtained by Affinity Capture: John LaCava , Javier Fernandez-Martinez, and Michael P. Rout
3 Density Gradient Ultracentrifugation to Isolate Endogenous Protein Complexes After Affinity Capture: Javier Fernandez-Martinez, John LaCava , and Michael P. Rout
CHAPTER 22 Proteomic Analysis of Protein Posttranslational Modifications by Mass Spectrometry
INTRODUCTION
Proteomic Analysis of Protein Posttranslational Modifications by Mass Spectrometry: Danielle L Swaney and Judit Villen
PROTOCOLS
1 Enrichment of Phosphopeptides via Immobilized Metal Affinity Chromatography: Danielle L Swaney and Judit Villen
2 Enrichment of Modified Peptides via Immunoaffinity Precipitation with Modification-Specific Antibodies: Danielle L Swaney and Judit Villen
CHAPTER 23 Protein Microarrays: Flexible Tools for Scientific Innovation
INTRODUCTION
Protein Microarrays: Flexible Tools for Scientific Innovation: Johnathan P Neiswinger, Ijeoma Uzoma, Eric Cox, HeeSool Rho, Guang Song, Corry Paul, Jun Seop Jeong, Kuan-Yi Lu, Chien-Sheng Chen, and Heng Zhu
PROTOCOLS
1 Characterization of Protein-Protein Interactions Using Protein Microarrays: Corry Paul, HeeSool Rho, Johnathan Neiswinger, and Heng Zhu
2 Characterization of RNA-Binding Proteins Using Protein Microarrays: Guang Song, Johnathan Neiswinger, and Heng Zhu
3 Characterization of Lipid-Protein Interactions Using Nonquenched Fluorescent Liposomal Nanovesicles and Yeast Proteome Microarrays: Kuan-Yi Lu, Chien-Sheng Chen, Johnathan Neiswinger, and Heng Zhu
4 Posttranslational Modification Assays on Functional Protein Microarrays: Johnathan Neiswinger, Ijeoma Uzoma, Eric Cox, HeeSool Rho, Jun Seop Jeong, and Heng Zhu
CHAPTER 24 Synthetic Genetic Arrays: Automation of Yeast Genetics
INTRODUCTION
Synthetic Genetic Arrays: Automation of Yeast Genetics: Elena Kuzmin, Michael Costanzo, Brenda Andrews, and Charles Boone
PROTOCOL
1 Synthetic Genetic Array Analysis: Elena Kuzmin, Michael Costanzo, Brenda Andrews, and Charles Boone
CHAPTER 25 Systematic Mapping of Chemical-Genetic Interactions in Saccharomyces cerevisiae
INTRODUCTION
Systematic Mapping of Chemical-Genetic Interactions in Saccharomyces cerevisiae: Sundari Suresh, Ulrich Schlecht, Weihong Xu, Walter Bray, Molly Miranda, Ronald W. Davis, Corey Nislow, Guri Giaever, R. Scott Lokey, and Robert P. St.Onge
PROTOCOLS
1 A High-Throughput Yeast Halo Assay for Bioactive Compounds: Walter Bray and R. Scott Lokey
2 Identification of Chemical-Genetic Interactions via Parallel Analysis of Barcoded Yeast Strains: Sundari Suresh, Ulrich Schlecht, Weihong Xu, Molly Miranda, Ronald W. Davis, Corey Nislow, Guri Giaever, and Robert P. St.Onge
CHAPTER 26 Prions
INTRODUCTION
Prions: Dmitry Kryndushkin, Herman K. Edskes, Frank P. Shewmaker, and Reed B. Wickner
PROTOCOLS
1 Genetic Methods for Studying Yeast Prions: Reed B. Wickner, Herman K. Edskes, Dmitry Kryndushkin, and Frank P. Shewmaker
2 Prion Transfection of Yeast: Herman K. Edskes, Dmitry Kryndushkin, Frank P. Shewmaker, and Reed B. Wickner
3 Isolation and Analysis of Prion and Amyloid Aggregates from Yeast Cells: Dmitry Kryndushkin, Natalia Pripuzova, and Frank P. Shewmaker
CHAPTER 27 Gene-Centered Yeast One-Hybrid Assays
INTRODUCTION
Gene-Centered Yeast One-Hybrid Assays: Juan I. Fuxman Bass, John S. Reece-Hoyes, and Albertha J.M. Walhout
PROTOCOLS
1 Generating Bait Strains for Yeast One-Hybrid Assays: Juan I. Fuxman Bass, John S. Reece-Hoyes, and Albertha J.M. Walhout
2 Performing Yeast One-Hybrid Library Screens: Juan I. Fuxman Bass, John S. Reece-Hoyes, and Albertha J.M. Walhout
3 Colony Lift Colorimetric Assay for β-Galactosidase Activity: Juan I. Fuxman Bass, John S. Reece-Hoyes, and Albertha J.M. Walhout
4 Zymolase-Treatment and Polymerase Chain Reaction Amplification from Genomic and Plasmid Templates from Yeast: Juan I. Fuxman Bass, John S. Reece-Hoyes, and Albertha J.M. Walhout
CHAPTER 28 Transposon Calling Cards
INTRODUCTION
Transposon Calling Cards: David Mayhew and Robi D. Mitra
PROTOCOL
1 Calling Card Analysis in Budding Yeast: David Mayhew and Rob D. Mitra
CHAPTER 29 Transcription Factor-DNA Binding Motifs in Saccharomyces cerevisiae: Tools and Resources
INTRODUCTION
Transcription Factor-DNA Binding Motifs in Saccharomyces cerevisiae: Tools and Resources: Joshua L. Schipper and Raluca M. Gordan
CHAPTER 30 The Saccharomyces Genome Database—A Tool for Discovery
INTRODUCTION
The Saccharomyces Genome Database—A Tool for Discovery: J. Michael Cherry

PROTOCOLS
1 The Saccharomyces Genome Database—Exploring Biochemical Pathways and Mutant Phenotypes: J. Michael Cherry
2 The Saccharomyces Genome Database—Advanced Searching Methods and Data Mining: J. Michael Cherry
3 The Saccharomyces Genome Database—Gene Product Annotation of Function, Process, and Component: J. Michael Cherry
4 The Saccharomyces Genome Database—Exploring Genome Features and Their Annotations: J. Michael Cherry
CHAPTER 31 BioGRID: A Tool for Studying Biological Interactions in Yeast
INTRODUCTION
BioGRID: A Tool for Studying Biological Interactions in Yeast: Rose Oughtred, Andrew Chatr-aryamontri, Bobby-Joe Breitkreutz, Christie S. Chang, Jennifer M. Rust, Chandra L. Theesfeld, Sven Heinicke, Ashton Breitkreutz, Daici Chen, Jodi Hirschman, Nadine Kolas, Michael S. Livstone, Julie Nixon, Lara O’Donnell, Lindsay Ramage, Andrew Winter, Teresa Reguly, Adnane Sellam, Chris Stark, Lorrie Boucher, Kara Dolinski, and Mike Tyers
PROTOCOL
1 Use of the BioGRID Database for Analysis of Yeast Protein and Genetic Interactions: Rose Oughtred, Andrew Chatr-aryamontri, Bobby-Joe Breitkreutz, Christie S. Chang, Jennifer M. Rust, Chandra L. Theesfeld, Sven Heinicke, Ashton Breitkreutz, Daici Chen, Jodi Hirschman, Nadine Kolas, Michael S. Livstone, Julie Nixon, Lara O’Donnell, Lindsay Ramage, Andrew Winter, Teresa Reguly, Adnane Sellam, Chris Stark, Lorrie Boucher, Kara Dolinski, and Mike Tyers
CHAPTER 32 Exploratory Analysis of Biological Networks Through Visualization, Clustering, and Functional Annotation in Cytoscape
PROTOCOL
Exploratory Analysis of Biological Networks Through Visualization, Clustering, and Functional Annotation in Cytoscape: Anastasia Baryshnikova
CHAPTER 33 Metabolomics in Yeast
INTRODUCTION
Metabolomics in Yeast: Amy A. Caudy, Michael Mulleder, and Markus Ralser
PROTOCOLS
1 Metabolite Extraction from Saccharomyces cerevisiae for Liquid Chromatography-Mass Spectrometry: Adam P. Rosebrock and Amy A. Caudy
2 A High-Throughput Method for the Quantitative Determination of Free Amino Acids in Saccharomyces cerevisiae by Hydrophilic Interaction Chromatography-Tandem Mass Spectrometry: Michael Mulleder, Katharina Bluemlein, and Markus Ralser
3 Spectrophotometric Analysis of Ethanol and Glucose Concentrations in Yeast Culture Media: Amy A. Caudy
CHAPTER 34 High-Throughput Yeast Strain Sequencing
INTRODUCTION
High-Throughput Yeast Strain Sequencing: Katja Schwartz and Gavin Sherlock
PROTOCOL
1 Preparation of Yeast DNA Sequencing Libraries: Katja Schwartz and Gavin Sherlock
CHAPTER 35 Budding Yeast Strains and Genotype-Phenotype Mapping
INTRODUCTION
Budding Yeast Strains and Genotype-Phenotype Mapping: Gianni Liti, Jonas Warringer, and Anders Blomberg
PROTOCOLS
1 Isolation and Laboratory Domestication of Natural Yeast Strains: Gianni Liti, Jonas Warringer, and Anders Blomberg
2 Mapping Quantitative Trait Loci in Yeast: Gianni Liti, Jonas Warringer, and Anders Blomberg
3 Yeast Reciprocal Hemizygosity to Confirm the Causality of a QTL-Associated Gene: Jonas Warringer, Gianni Liti, and Anders Blomberg
CHAPTER 36 Genetic Analysis of Complex Traits in Saccharomyces cerevisiae
INTRODUCTION
Genetic Analysis of Complex Traits in Saccharomyces cerevisiae: Ian M. Ehrenreich and Paul M. Magwene
PROTOCOL
Genetic Dissection of Heritable Traits in Yeast Using Bulk Segregant Analysis: Ian M. Ehrenreich and Paul M. Magwene
CHAPTER 37 Chemostat Culture for Yeast Physiology and Experimental Evolution
INTRODUCTION
Chemostat Culture for Yeast Physiology and Experimental Evolution: Maitreya J. Dunham, Emily O. Kerr, Aaron W. Miller, and Celia Payen
PROTOCOLS
1 Assembly of a Mini-Chemostat Array: Aaron W. Miller, Emily O. Kerr, and Maitreya J. Dunham
2 Chemostat Culture for Yeast Physiology: Emily O. Kerr and Maitreya J. Dunham
3 Chemostat Culture for Yeast Experimental Evolution: Celia Payen and Maitreya J. Dunham
CHAPTER 38 Methods to Synthesize Large DNA Fragments for a Synthetic Yeast Genome
PROTOCOL
Methods to Synthesize Large DNA Fragments for a Synthetic Yeast Genome: Yizhi Cai and Junbiao Dai
APPENDIX General Safety and Hazardous Material Information
INDEX

Reviews

“With the explosive growth in biotechnology in the new millennium, finding a manual that manages to be simultaneously authoritative, up to date, comprehensive, and instructive is a rarity. Luckily for those of us working on the budding yeast, Saccharomyces cerevisiae, we have just such a book with Budding Yeast: A Laboratory Manual.”
—The Quarterly Review of Biology

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Budding Yeast: A Laboratory Manual [Paperback]

Budding Yeast: A Laboratory Manual

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