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Subject Area(s):  Microscopy and ImagingCell Biology

Edited by Sandra L. Schmid, University of Texas Southwestern Medical Center; Alexander Sorkin, University of Pittsburgh School of Medicine; Marino Zerial, Max Planck Institute of Molecular and Cell Biology

© 2014 • 590 pp., illus., index
Hardcover •
ISBN  978-1-621820-24-6

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  •     Description    
  •     Contents    


During endocytosis, extracellular molecules and plasma membrane components are selectively internalized by cells. This fundamental process of “cellular ingestion” is required for diverse activities such as nutrient uptake, cell adhesion and migration, signal transduction, cytokinesis, neurotransmission, and antigen presentation. Pathogens (e.g., HIV) exploit endocytic pathways to gain entry into cells, and defects in the endocytic machinery can lead to diseases such as cancer.

Written and edited by experts in the field, this collection from Cold Spring Harbor Perspectives in Biology covers all of the major pathways of endocytosis and postendocytic trafficking and how they regulate cellular and organismal physiology. Contributors describe how cargo enters the cell via clathrin-mediated and clathrin-independent pathways, including caveolar endocytosis, micropinocytosis, cholesterol-sensitive endocytosis, phagocytosis, and the CLIC/GEEC pathway. They review the numerous machineries (e.g., Rab GTPases, tethering factors, and retromer) that transport cargo through endosomes and deliver it to lysosomes or recycle it back to the cell surface, and the signals and mechanisms governing these sorting decisions. Topics such as lysosomal dynamics, the biophysical challenges of bending membranes, and the evolution of endocytic systems are also covered.

This volume also includes substantial discussion of the roles of endocytic trafficking in organismal development, physiology, and disease. It is thus an indispensable reference for cell biologists, but also for neuroscientists, immunologists, developmental biologists, microbiologists, and others concerned with the physiological and therapeutic implications of this key cellular process.


Endocytosis: Past, Present, and Future
Sandra L. Schmid, Alexander Sorkin, and Marino Zerial
Molecular Structure, Function, and Dynamics of Clathrin-Mediated Membrane Traffic
Tom Kirchhausen, David Owen, and Stephen C. Harrison
Endocytic Accessory Factors and Regulation of Clathrin-Mediated Endocytosis
Christien J. Merrifield and Marko Kaksonen
Cargo Recognition in Clathrin-Mediated Endocytosis
Linton M. Traub and Juan S. Bonifacino
Imaging and Modeling the Dynamics of Clathrin-Mediated Endocytosis
Marcel Mettlen and Gaudenz Danuser
Clathrin-Independent Pathways of Endocytosis
Satyajit Mayor, Robert G. Parton, and Julie G. Donaldson
The Complex Ultrastructure of the Endolysosomal System
Judith Klumperman and Graça Raposo
Rab Proteins and the Compartmentalization of the Endosomal System
Angela Wandinger-Ness and Marino Zerial
Retromer: A Master Conductor of Endosome Sorting
Christopher Burd and Peter J. Cullen
Lipid Sorting and Multivesicular Endosome Biogenesis
Christin Bissig and Jean Gruenberg
Molecular Mechanisms of the Membrane Sculpting ESCRT Pathway
William Mike Henne, Harald Stenmark, and Scott D. Emr
Ubiquitin-Dependent Sorting in Endocytosis
Robert C. Piper, Ivan Dikic, and Gergely Lukacs
The Biogenesis of Lysosomes and Lysosome-Related Organelles
J. Paul Luzio, Yvonne Hackmann, Nele M.G. Dieckmann, and Gillian M. Griffiths
Bending “On the Rocks”—A Cocktail of Biophysical Modules to Build Endocytic Pathways
Ludger Johannes, Christian Wunder, and Patricia Bassereau
Function and Regulation of the Endosomal Fusion and Fission Machineries
Alexis Gautreau, Ksenia Oguievetskaia, and Christian Ungermann
Lysosomal Adaptation: How the Lysosome Responds to External Cues
Carmine Settembre and Andrea Ballabio
Endocytosis and Autophagy: Exploitation or Cooperation?
Sharon A. Tooze, Adi Abada, and Zvulun Elazar
The Cell Biology of the Endocytic System from an Evolutionary Perspective
Jeremy G. Wideman, Ka Fai Leung, Mark C. Field, and Joel B. Dacks
Unconventional Functions for Clathrin, ESCRTs, and Other Endocytic Regulators in the Cytoskeleton, Cell Cycle, Nucleus, and Beyond: Links to Human Disease
Frances M. Brodsky, R. Thomas Sosa, Joel A. Ybe, and Theresa J. O’Halloran
Reciprocal Regulation of Endocytosis and Metabolism
Costin N. Antonescu, Timothy E. McGraw, and Amira Klip
Presynaptic Membrane Retrieval and Endosome Biology: Defining Molecularly Heterogeneous Synaptic Vesicles
Jennifer R. Morgan, Heather Skye Comstra, Max Cohen, and Victor Faundez
Neuronal Signaling through Endocytosis
Katharina E. Cosker and Rosalind A. Segal
MHC Class II Antigen Presentation by Dendritic Cells Regulated through Endosomal Sorting
Toine ten Broeke, Richard Wubbolts, and Willem Stoorvogel
Endocytosis, Signaling, and Beyond
Pier Paolo Di Fiore and Mark von Zastrow
Endocytosis and Signaling during Development
Christian Bökel and Michael Brand
Cargo Sorting in the Endocytic Pathway: A Key Regulator of Cell Polarity and Tissue Dynamics
Suzanne Eaton and Fernando Martin-Belmonte
The Role of Endocytosis during Morphogenic Signaling
Marcos Gonzales-Gaitan and Frank Jülicher
Role of Endosomes and Lysosomes in Human Disease
Frederick R. Maxfield
Endocytosis and Cancer
Ira Mellman and Yosef Yarden
Endocytosis of Viruses and Bacteria
Pascale Cossart and Ari Helenius
Exploiting Endocytosis for Nanomedicines
Akin Akinc and Giuseppe Battaglia
Imaging the Dynamics of Endocytosis in Live Mammalian Tissues
Roberto Weigert