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Genes & Development

Edited By Laureen Connell, Cold Spring Harbor Laboratory Press

Online Features: Genes and Development Online  – Internet edition

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© 2024 • ISSN 0890-9369; Volume 38 includes full online access
FREE TRIAL ACCESS offer for libraries! Contact [email protected].
January - December 2024, 24 monthly issues
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Description

Genes & Development publishes high-quality research papers of broad general interest and biological significance in the areas of molecular biology, molecular genetics, and related fields. G&D publishes two research formats; Research papers and short Research Communications, in addition to Review Articles and Perspectives. G&D consistently ranks in the top 10 among primary research journals in all fields of science.
This journal is published in association with The Genetics Society.
Subscription to the print journal includes full access to the online edition (online-only subscriptions are available upon request).

 
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Genome Research

Edited By Hillary Sussman, Cold Spring Harbor Laboratory Press; Aravinda Chakravarti, NYU Langone Health; Richard Gibbs, Baylor College of Medicine; Eric Green, Bethesda, MD; Richard Myers, HudsonAlpha Institute for Biotechnology; Evan Eichler, University of Washington; Anne Ferguson-Smith, University of Cambridge; Kateryna Makova, Penn State University

Online Features: Internet edition – Genome Research Online at Stanford University Libraries' HighWire Press; Electronic supplementary material – auxiliary data available only via the journal's online edition

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© 2024 • ISSN: 1088-9051; Volume 34 includes full online access
FREE TRIAL ACCESS offer for libraries! Contact [email protected].
January - December 2024, 12 monthly issues
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Description

In August 1995, Cold Spring Harbor Laboratory Press began publication of a new, monthly, international, peer-reviewed journal, Genome Research. The journal focuses on genome studies in all species, and presents research that provides or aids in genome-based analyses of biological processes. The journal represents a nexus point where genomic information, applications, and technology come together with biological information to create a more global understanding of all biological systems.

Among the research considered by the journal are novel reports on gene discovery, comparative genome analyses, evolution studies, informatics, genome structure and function, technological innovations and applications, statistical and mathematical methods, cutting-edge genetic and physical mapping and DNA sequencing, and other reports that present data where sequence information is used to address biological concerns.

New data in these areas are published as research papers in the form of articles and letters, or methods and resource reports that provide novel information on methodologies or resources that will be of interest to a broad readership. Complete data sets are presented electronically on the journal's web site where appropriate. The journal also provides review articles, perspectives, and Insight/Outlook articles, which presents commentary on the latest advances published both here and elsewhere, placing such progress in its broader biological context.

Subscription to the print journal includes full access to the online edition (online-only subscriptions are not available).

 
 

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Learning & Memory

Edited By John H. Byrne, University of Texas School of Medicine

Online Features: Internet edition – Learning & Memory Online at Stanford University Libraries' HighWire Press

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© 2024 • ISSN 1549-5485; Volume 31; includes full online access
FREE TRIAL ACCESS offer for libraries! Contact [email protected].
January - December 2024, 12 online  issues
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Learning & Memory is the journal of choice for interdisciplinary studies on synaptic plasticity, learning, and memory. Until now, there has been no one journal devoted to this area of study and no dominant journal that demands attention by serious workers in the area, regardless of specialty. Spanning molecules, cells, systems, and behavior, L&M considers research using approaches from cellular and molecular biology, genetics, biochemistry, computational neuroscience, neurophysiology, neuropharmacology, neuroanatomy, behavior, and cognition. Learning & Memory provides a forum for these investigations in the form of original research papers, review articles, and brief communications.

 
 

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Genome Research [Journal]
RNA [Journal]
 
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CSH Perspectives in Medicine

Online Features: CSH Perspectives in Medicine – CSH Perspectives in Medicine at Stanford University Libraries' HighWire Press

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© 2024 • ISSN: 2157-1422; Volume 14 includes full online access
FREE TRIAL ACCESS offer for libraries! Contact
[email protected].

January - December 2024, 12 monthly issues
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Description

Cold Spring Harbor Perspectives in Medicine is a new online publication covering everything from the molecular and cellular bases of disease to translational medicine and new therapeutic strategies. Each issue offers reviews on different aspects of a variety of diseases and the tissues they affect. The contributions are written by experts in each field and commissioned as Subject Collections by a board of eminent scientists and physicians, all of whom are acknowledged to be key figures in their particular fields. These Subject Collections gradually accumulate articles as new issues of the journal are published and, when complete, each represents a comprehensive survey of the field it covers. Cold Spring Harbor Perspectives in Medicine is thus unmatched in its depth of coverage and represents an essential source where readers can find informed surveys and critical discussion of advances in molecular medicine.

Please visit www.perspectivesinmedicine.org for complete information about the journal.

Subject Coverage includes:

  • Addiction
  • Aging
  • Alzheimer Disease
  • Anemia
  • Antibiotic Resistance
  • Bacterial Pathogens
  • Bone Disease
  • Cancer
  • Cystic Fibrosis
  • Diabetes
  • Heart Disease
  • Hemoglobin
  • HIV
  • Influenza
  • Malaria
  • Multiple Sclerosis
  • Muscular Dystrophy
  • Parkinson's Disease
  • Prion Diseases
  • Skin Diseases
  • Schizophrenia
 
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CSH Perspectives in Biology

Online Features: CSH Perspectives in Biology – CSH Perspectives in Biology at Stanford University Libraries' HighWire Press

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© 2024 • ISSN: 1943-0264; Volume 16 includes full online access
FREE TRIAL ACCESS offer for libraries! Contact
[email protected].

January - December 2024, 12 monthly issues
Journal Subscription

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Description

A new online publication for the molecular life sciences

Cold Spring Harbor Perspectives in Biology provides the scientific community with comprehensive, systematic surveys of topics in the molecular life sciences where research is breaking new ground

In July 2009, Cold Spring Harbor Laboratory Press launched a new monthly journal, Cold Spring Harbor Perspectives in Biology, that provides comprehensive, systematically structured surveys of research in exciting areas of molecular and cellular biology, genetics, developmental biology, neuroscience, cancer biology, and molecular pathology.

For decades, Cold Spring Harbor Laboratory has complemented its groundbreaking research with publication of a variety of highly prestigious titles written and edited by leading scientists. These include the journals Genes & Development and Genome Research, the bible of research methods, Molecular Cloning, online teaching resources, and numerous other laboratory manuals, monographs, handbooks, journals, and textbooks.

Cold Spring Harbor Perspectives in Biology (http://www.cshperspectives.org; ISSN 1943-0264) is the latest, and perhaps most ambitious, addition to this stable. Its depth of coverage is unmatched, spanning the entire spectrum of the molecular life sciences and therefore providing an essential resource for all areas of bioscience research.

According to the journal's Executive Editor, Richard Sever, who has worked on traditional review journals including the Current Opinion and Trends titles, Cold Spring Harbor Perspectives in Biology has a new and unique approach. "There are many review articles published each year, but they're usually on one particular aspect of one particular field," he says. "CSH Perspectives is different. We cover subjects by asking experts to consider how best to structure a comprehensive survey of their field, and then commission articles from all the key figures in that field. And we cover everything in bioscience from Alzheimer's to plant biology."

CSH Perspectives articles are all commissioned as �collections� by a board of eminent academic editors and gradually accumulate online at the journal's website each month as they are accepted and published. The debut issue features articles commissioned by Elaine Fuchs, Michael Karin, W. James Nelson, and Peter Lawrence and includes contributions from Nobel laureate David Baltimore, molecular motor expert Nobutaka Hirokawa, and developmental biologist Jim Smith. These set the stage for subject collections on symmetry breaking, morphogen gradients, cell junctions, and the immune signaling molecule NF-kB. Next in the pipeline are articles on the origin of life, the p53-family of tumor suppressors, the nucleus, and immune tolerance.

The new publication is produced online in partnership with Stanford University Library's HighWire Press, taking advantage of their new H20 platform. The state-of-the-art website includes HTML and PDF views, high-resolution artwork, and video material, along with a host of novel features such as author updates, commenting options, and Twitter-powered news feeds.

 
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CSH Protocols

Edited By Alejandro Montenegro-Montero, Cold Spring Harbor Laboratory Press

Online Features: Internet edition – Cold Spring Harbor Protocols at Stanford University Libraries' HighWire Press

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© 2024 • ISSN: 1559-6095; Volume 19; includes full online access
FREE TRIAL ACCESS offer for libraries! Contact [email protected].
January - December 2024, 12 monthly issues
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Description

  • The online source of trusted techniques in molecular and cellular biology
  • Contains new and classic protocols presented step-by-step with recipes and troubleshooting
  • Frequently updated and annotated
  • Interactive, customizable, and fully searchable

The development of CSH Protocols is a response to requests from scientists for authoritative information about techniques with a broad editorial scope, delivered through a state-of-the-art online interface. At this site, you will find methods from Molecular Cloning along with selected protocols from many of our best-selling manuals, such as Cells and Antibodies, as well as protocols from Cold Spring Harbor's renowned on-site courses. In addition, you will discover new cutting-edge protocols submitted by and commissioned from laboratories worldwide. A peer-reviewed online journal, CSH Protocols is updated monthly.

Features include:
  • Step-by-step, uniformly structured formats for ease of use and printing, with clearly linked materials, cautions, recipes, and troubleshooting
  • Customizable, topic-based e-mail alerts for the latest updates in a particular field
  • Personal folders for storing favorite protocols and searches
  • Navigation tools including a specifically designed taxonomy for browsing and a variety of full-text search options
  • A kit search tool for fast access to time-saving resources
  • An online manuscript submission system for authors to contribute new protocols

CSH Protocols — More Than Just Methods.

Visit www.cshprotocols.org for more details on features, subscriptions, and manuscript submission.

  • Available exclusively via institutional site license
  • ISSN 1559-6095 / online, monthly

Read within Top Institutions and Companies Such as:

JANELIA FARM RESEARCH CAMPUS
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  • Stem Cells
  • Transgenic Technology
 
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RNA

Edited By Timothy W. Nilsen, Editor-in-Chief, Case Western Reserve University; Thomas R. Cech, Howard Hughes Medical Institute; David Engelke, University of Michigan; Eric Westhof, CNRS Strasbourg; Marvin Wickens, University of Wisconsin

Online Features: Internet edition – RNA Online at Stanford University Libraries' HighWire Press

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© 2024 • ISSN 1355-8382; Volume 30; includes full online access.
FREE TRIAL ACCESS offer for libraries! Contact [email protected].
(Published by CSHL Press for The RNA Society)
January - December 2024, 12 monthly issues
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Description

RNA, the publication of the RNA Society, publishes high quality research papers that provide significant new insight into any area of RNA biology, including molecular biology, cell biology, biochemistry, bioinformatics, genetics and evolution. RNA publishes two research formats, articles and short reports. In addition, the journal publishes novel methodologies, reviews and perspectives. RNA provides a unique resource to all members of the RNA community and those that wish to keep abreast of this quickly moving area.

 
 

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The Medical Revolution of Messenger RNA


Subject Area(s):  General Interest TitlesViruses

By Fabrice Delaye

Download a Free Excerpt from The Medical Revolution of Messenger RNA:

Foreword
Introduction
Index


Due October 17, 2023 • 136 pages, illustrated (16 B&W), index + 8-page insert
Hardcover • $29.50 26.55
ISBN  978-1-621824-94-7
You save: 10%

  •     Description    
  •     Contents    
  •     Reviews    
  •     Related Titles    

Description

To the casual observer, it took just 10 months to develop a vaccine against the virus responsible for the COVID-19 pandemic. That extraordinary success was made possible by the use of messenger RNA (mRNA), the molecule that instructs cells to make a virus surface protein that stimulates the production of antiviral antibodies.

What most people don’t know is that this apparently breakthrough technology had been in development for three frustrating decades. And that it was preceded by 30 earlier years of fundamental research.

This book tells the story of how mRNA’s medical potential was finally realized, setting the stage for a coming revolution in which our own bodies will generate therapeutic molecules we need. mRNA was long overlooked by mainstream molecular biologists. The pathway to recognition of its therapeutic possibilities was littered with broken careers, lawsuits, and opportunities missed by pharmaceutical companies. For the scientists who persisted through years of academic and commercial disappointment, the COVID-19 vaccine was an enormous vindication and an important step toward a new generation of therapies.

Fabrice Delaye, a science and technology reporter for Heidi.news in Switzerland, interviewed more than 40 scientists and entrepreneurs worldwide to create this definitive account of the rise of a technology that promises to revolutionize medicine.

FABRICE DELAYE, 58, is a science and technology journalist based in Switzerland. He was U.S. correspondent at the daily Swiss newspaper L’Agefi, science and technology editor at magazine Bilan, and is now a reporter-at-large for Heidi.news in Geneva. He is a graduate of the Institut d'Études Politiques de Paris and has a master's degree from the Swiss Institute of Technology in Lausanne, EPFL.

When COVID-19 imposed its relentless grip on the global population and messenger RNA–based vaccines came to the rescue in seemingly record time, Delaye realized that their development could not have been as simple and quick as people wanted to believe. But it was not until he went in pursuit of the origins of mRNA technologies that he uncovered a dramatic story that had never been told.

Maximizing his decades of contacts and his unique grasp of the science and the stakes involved, Delaye set out to document the long, harrowing, unlikely but ultimately triumphant road to discovery of RNA-based technology with the potential to transform the world far beyond the pandemic.

Contents

Foreword, by Thomas R. Cech
Acknowledgments
Introduction: What Happens When the Jab Goes into Your Arm?
Prologue: 1960–1990: The Beatles Years of mRNA
1 From Science to Technology at the Salk Institute
2 RNA or DNA? How Merck Missed the Jackpot
3 How French RNA Pioneers Killed the Goose That Laid the Golden Eggs
4 How Karikó and Weissman Wove an Invisibility Cloak
5 CureVac: How the German Hare Became a Tortoise
6 Moderna's Moonshot
7 The Race for mRNA Vaccines Told by Its Referee
8 BioNTech: What’s Next?
9 Lipids: The Key to the Third Medical Revolution
Glossary
Timeline
Selected Bibliography
Index
Photo Section

Reviews

review:  “The fascinating story of mRNA vaccine development reads like a thriller. It highlights the human dimension of an extraordinary achievement.”
      —PATRICK AEBISCHER, MD
President Emeritus of the Swiss Institute of Technology in Lausanne (EPFL)

review:  “The 327 days it took to make the mRNA COVID-19 vaccines changed the world of science; Fabrice Delaye’s book tells the fascinating inside story of the many years of scientific endeavor it took to make it happen.”
      —SETH BERKLEY, MD
CEO of Gavi, the Vaccine Alliance; Co-founder of COVAX

   

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Mouse Phenotypes: Generation and Analysis of Mutants, Second Edition: A Laboratory Manual


Subject Area(s):  Developmental BiologyBiotechnologyGenetics

By Virginia E. Papaioannou; Richard R. Behringer

Download a Free Excerpt from Mouse Phenotypes: Generation and Analysis of Mutants, Second Edition: A Laboratory Manual:

Preface
Chapter 3. Mouse Gene-Targeting Strategies for Maximum Ease and Versatility
Index


Due November 2023 • 296 pages, illustrated (78 color and 60 B&W), index
Paperback • $95
ISBN  978-1-621824-18-3

This title also available in: Hardcover

  •     Description    
  •     Contents    

Description

In this postgenome era, the large number of mouse mutations available is revolutionizing biomedical research. Large-scale mutagenesis projects have resulted in the modification of thousands of mouse genes and, more recently, with CRISPR–Cas gene editing, generation of custom mutations is easier than ever. The interpretation of mutant phenotypes is critically important for understanding how changes in the genome result in changes in the phenotype. Complex phenotypes resulting from gene manipulation or spontaneous mutation, however, provide a huge analytic challenge. Where does one start with the analysis? How does one dissect the phenotype and begin to understand the molecular and developmental consequences of the genotype? How does one make the best use of the valuable resource that you have developed?

Mouse Phenotypes: A Handbook of Mutation Analysis, Second Edition, provides a road map for such analysis. Advice and practical guidance are provided, starting with the planning stages of gene targeting, CRISPR–Cas gene editing, or mutagenesis experiments, through the complete analysis of complex phenotypes, including tips on breeding strategies, studying dominant mutations, and characterizing a mutant mouse with no apparent phenotypic abnormalities. A systematic approach to the analysis, complemented with simple protocols, will help users make a comprehensive assessment of a particular phenotype resulting from a mutation and determine the timing and likely causes of specific developmental failures. Investigators using the laboratory mouse in genetic studies designed to understand mammalian biology will benefit from the authors’ experience and advice in analyzing mutant phenotypes.

Contents

Preface
Chapter 1. Strategies for the Production and Phenotypic Analysis of Mutations in the Mouse
Chapter 2. Obtaining or Generating Gene Mutations in Mice
Chapter 3. Mouse Gene-Targeting Strategies for Maximum Ease and Versatility
Chapter 4. Embryonic Stem Cell Gene Targeting and Chimera Production in Mice
PROTOCOLS
Protocol 4.1 Counting Murine Corpora Lutea to Determine the Number of Oocytes Ovulated
Protocol 4.2 Cross-Fostering of Newborn Mice to Counteract Bad Parenting
Chapter 5. Recovering a Targeted Mutation in Mice from Embryonic Stem Cell Chimeras or CRISPRCas Founders
PROTOCOL
Protocol 5.1 Sex Genotyping Mice by Polymerase Chain Reaction
Chapter 6. Strategies for Maintaining Mouse Mutations
PROTOCOL
Protocol 6.1 Backcrossing to Generate a Congenic Mouse Strain
Chapter 7. Special Breeding Techniques for Use in Mouse Mutation Analysis
Chapter 8. Phenotypic Analysis: Assessing Timing of Recessive Prenatal Lethality in Mice
PROTOCOL
Protocol 8.1 Establishing Control Frequency of Embryonic and Fetal Loss in a Mutant Mouse Colony
Chapter 9. Phenotypic Analysis of Preimplantation Lethality in Mice
PROTOCOLS
Protocol 9.1 Phenotypic Analysis of Preimplantation Mouse Embryos in Culture
Protocol 9.2 Cell Counting Techniques for Preimplantation Mouse Embryos Using Fluorescent DNA Dyes
Chapter 10. Phenotypic Analysis of Periimplantation to Mid-Gestation Lethality in Mic
PROTOCOL
Protocol 10.1 Orientation of Implanted or Dissected Mouse Embryos for Histological Sectioning
Chapter 11. Analysis of Mid- to Late-Gestation Phenotypes in Mice
Chapter 12. Uncovering Phenotypes in Mutant Mice by Determining Embryo, Organ, Tissue, and Cell Developmental Potential
Chapter 13. Phenotypic Analysis of Perinatal Lethality in Mice
Chapter 14. Analysis of Postnatal Mutant Phenotypes in Mice
Chapter 15. The No Phenotype Challenge in Analyzing Mutant Mice
PROTOCOL
Protocol 15.1 Necropsy Guide for the Collection of Tissues from Mice with or without Tumors
Chapter 16. Phenotypic Analysis of Dominant Mutant Effects in Mice
PROTOCOL
Protocol 16.1 Isolation of XO Subclones from XY Murine Embryonic Stem Cells
Chapter 17. Getting around an Early Lethal Phenotype in Mice with Chimeras
Chapter 18. Tissue- and/or Temporal-Specific Mutations in Mice Using Conditional Alleles
APPENDIXES
1. Useful Books and Other Resources
2. Examples of Phenotype Analysis
3. Glossary
4. General Safety and Hazardous Material Information
Index
   

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Add To Cart

Mouse Phenotypes: Generation and Analysis of Mutants, Second Edition: A Laboratory Manual


Subject Area(s):  Developmental BiologyBiotechnologyGenetics

By Virginia E. Papaioannou; Richard R. Behringer

Download a Free Excerpt from Mouse Phenotypes: Generation and Analysis of Mutants, Second Edition: A Laboratory Manual:

Preface
Chapter 3. Mouse Gene-Targeting Strategies for Maximum Ease and Versatility
Index


Due November 2023 • 296 pages, illustrated (78 color and 60 B&W), index
Hardcover • $165
ISBN  978-1-621824-17-6

This title also available in: Paperback

  •     Description    
  •     Contents    

Description

In this postgenome era, the large number of mouse mutations available is revolutionizing biomedical research. Large-scale mutagenesis projects have resulted in the modification of thousands of mouse genes and, more recently, with CRISPR–Cas gene editing, generation of custom mutations is easier than ever. The interpretation of mutant phenotypes is critically important for understanding how changes in the genome result in changes in the phenotype. Complex phenotypes resulting from gene manipulation or spontaneous mutation, however, provide a huge analytic challenge. Where does one start with the analysis? How does one dissect the phenotype and begin to understand the molecular and developmental consequences of the genotype? How does one make the best use of the valuable resource that you have developed?

Mouse Phenotypes: A Handbook of Mutation Analysis, Second Edition, provides a road map for such analysis. Advice and practical guidance are provided, starting with the planning stages of gene targeting, CRISPR–Cas gene editing, or mutagenesis experiments, through the complete analysis of complex phenotypes, including tips on breeding strategies, studying dominant mutations, and characterizing a mutant mouse with no apparent phenotypic abnormalities. A systematic approach to the analysis, complemented with simple protocols, will help users make a comprehensive assessment of a particular phenotype resulting from a mutation and determine the timing and likely causes of specific developmental failures. Investigators using the laboratory mouse in genetic studies designed to understand mammalian biology will benefit from the authors’ experience and advice in analyzing mutant phenotypes.

Contents

Preface
Chapter 1. Strategies for the Production and Phenotypic Analysis of Mutations in the Mouse
Chapter 2. Obtaining or Generating Gene Mutations in Mice
Chapter 3. Mouse Gene-Targeting Strategies for Maximum Ease and Versatility
Chapter 4. Embryonic Stem Cell Gene Targeting and Chimera Production in Mice
PROTOCOLS
Protocol 4.1 Counting Murine Corpora Lutea to Determine the Number of Oocytes Ovulated
Protocol 4.2 Cross-Fostering of Newborn Mice to Counteract Bad Parenting
Chapter 5. Recovering a Targeted Mutation in Mice from Embryonic Stem Cell Chimeras or CRISPRCas Founders
PROTOCOL
Protocol 5.1 Sex Genotyping Mice by Polymerase Chain Reaction
Chapter 6. Strategies for Maintaining Mouse Mutations
PROTOCOL
Protocol 6.1 Backcrossing to Generate a Congenic Mouse Strain
Chapter 7. Special Breeding Techniques for Use in Mouse Mutation Analysis
Chapter 8. Phenotypic Analysis: Assessing Timing of Recessive Prenatal Lethality in Mice
PROTOCOL
Protocol 8.1 Establishing Control Frequency of Embryonic and Fetal Loss in a Mutant Mouse Colony
Chapter 9. Phenotypic Analysis of Preimplantation Lethality in Mice
PROTOCOLS
Protocol 9.1 Phenotypic Analysis of Preimplantation Mouse Embryos in Culture
Protocol 9.2 Cell Counting Techniques for Preimplantation Mouse Embryos Using Fluorescent DNA Dyes
Chapter 10. Phenotypic Analysis of Periimplantation to Mid-Gestation Lethality in Mic
PROTOCOL
Protocol 10.1 Orientation of Implanted or Dissected Mouse Embryos for Histological Sectioning
Chapter 11. Analysis of Mid- to Late-Gestation Phenotypes in Mice
Chapter 12. Uncovering Phenotypes in Mutant Mice by Determining Embryo, Organ, Tissue, and Cell Developmental Potential
Chapter 13. Phenotypic Analysis of Perinatal Lethality in Mice
Chapter 14. Analysis of Postnatal Mutant Phenotypes in Mice
Chapter 15. The No Phenotype Challenge in Analyzing Mutant Mice
PROTOCOL
Protocol 15.1 Necropsy Guide for the Collection of Tissues from Mice with or without Tumors
Chapter 16. Phenotypic Analysis of Dominant Mutant Effects in Mice
PROTOCOL
Protocol 16.1 Isolation of XO Subclones from XY Murine Embryonic Stem Cells
Chapter 17. Getting around an Early Lethal Phenotype in Mice with Chimeras
Chapter 18. Tissue- and/or Temporal-Specific Mutations in Mice Using Conditional Alleles
APPENDIXES
1. Useful Books and Other Resources
2. Examples of Phenotype Analysis
3. Glossary
4. General Safety and Hazardous Material Information
Index
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