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Binding and Kinetics for Molecular Biologists


Subject Area(s):  Molecular BiologyBiochemistryHandbooksLaboratory Techniques

By James A. Goodrich, University of Colorado, Boulder; Jennifer F. Kugel, University of Colorado, Boulder

Online Features: Website

This companion website provides a set of computer simulations that can be used to visualize important concepts presented in the book, offering a better understanding of binding and rate curves and fitting data - http://kinetics.cshl.edu

View sample pages from the book here


© 2007 • 182 pp., illus., appendices, index
Paperback • $39 23.40
ISBN  978-1-621820-79-6
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  •     Description    
  •     Contents    
  •     Reviews    
  •     Related Titles    

Description

This handbook offers a practical guide to the principles of quantitative analysis in biological experiments. The material is primarily aimed at working molecular biologists, but the scope and clarity of presentation make it equally suitable as an introduction for students. Topics covered range from the basics—such as measuring the concentrations of macromolecules—through considerations of binding constants and the kinetics of molecular interactions. The book ends with a thorough consideration of data analysis.

Contents

1. Performing Quantitative Experiments with Biomolecules
2. Affinity Constants
3. Cooperativity in Binding
4. Rate Constants for Binding and Dissociation
5. Kinetics of Regulation and Cooperativity in Binding Reactions
6. Constants for a Two-step Binding Reaction
7. Data Analysis
Appendix 1: Equation Derivations
Appendix 2: Resources and References
Index

Reviews

review:  “This textbook provides a good introduction to the theory of binding kinetics. It supplies technical insight to proper affinity assay development. The volume is ideally suited for readers who seek background information prior to designing ligand-protein interaction assays.”
      —The Quarterly Review of Biology

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